TY - CHAP
T1 - Isolation and culture of oligodendrocytes
AU - Weil, Marie Theres
AU - Schulz-Ëberlin, Giselheid
AU - Mukherjee, Chaitali
AU - Kuo-Elsner, Wen Ping
AU - Schäfer, Isabelle
AU - Müller, Christina
AU - Simons, Mikael
N1 - Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.
PY - 2019
Y1 - 2019
N2 - Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.
AB - Primary cultures of brain-derived rodent cells are widely used to study molecular and cellular mechanisms in neurobiology. In this chapter, we describe methods of purifying and culturing oligodendroglial cells from mouse perinatal brains. In addition, we describe methods of coculturing the purified oligodendrocytes with neurons. When prepared and cultured according to these protocols, many essential aspects of the biology of oligodendrocytes, such as their proliferation, differentiation, and myelination, can be studied in culture.
KW - Coculture
KW - Magnetic-activated cell sorting
KW - Oligodendrocyte differentiation
KW - Oligodendrocyte precursor cells
KW - Oligodendrocyte primary culture
UR - https://www.scopus.com/pages/publications/85062765834
U2 - 10.1007/978-1-4939-9072-6_5
DO - 10.1007/978-1-4939-9072-6_5
M3 - Chapter
C2 - 30820894
AN - SCOPUS:85062765834
T3 - Methods in Molecular Biology
SP - 79
EP - 95
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -