Isolated microspore derived plant formation via embryogenesis in Triticum aestivum L.

S. K. Datta, G. Wenzel

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75 Scopus citations

Abstract

Embryogenesis in isolated microspores of wheat (Triticum aestivum L.) leading to plant regeneration has been established on modified liquid N6 medium (supplemented with 2,4-D, casein hydrolysate and Ficoll). Globular embryoids which were obtained after 6-8 weeks of culture of competent embryogenic microspores produced perfect embryoids when transferred to regeneration medium. Embryoids were differentiated to plants on other modified N6 agar medium (0.75% w/v agar, 20 g/l sucrose, 1 g/l myo-inositol, 8.8 μM 6-benzylaminopurine (BAP), 11.4 μM indoleacetic acid (IAA), 160 mg/l glutamine, 10 mg/l proline). Responses of microspores in regeneration and embryoid differentiation varied depending on the constituents of the media and genotypes used.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalPlant Science
Volume48
Issue number1
DOIs
StatePublished - 1987
Externally publishedYes

Keywords

  • Triticum aestivum
  • embryogenesis
  • haploids
  • microspore culture

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