Iodine species and the endocrine system: Thyroid hormone levels in adult Danio rerio and developing Xenopus laevis

R. Simon, J. E. Tietge, B. Michalke, S. Degitz, K. W. Schramm

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS). This method enables quantification of iodide, T4 and T3, as well as reverse T3 (rT3) and the synthetic precursors of TH, monoiodotyrosine (MIT), and diiodotyrosine (DIT) in a single injection. In this work, the LC-ICP-MS approach was used to analyze whole-body homogenates of adult male and female zebrafish (Danio rerio) and tadpoles of the African clawed frog (Xenopus laevis) at two different developmental stages (NF58 and 61) according to Nieuwkoop and Faber. The data demonstrate that the LC-ICP-MS method was successful at measuring I-, MIT, DIT, T4, T3, and rT3 in these two species. Furthermore, the method also detected five additional iodinated compounds which are currently unidentified.

Original languageEnglish
Pages (from-to)481-485
Number of pages5
JournalFresenius' Journal of Analytical Chemistry
Volume372
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Keywords

  • Iodine species
  • Thyroid
  • Zebrafish

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