TY - JOUR
T1 - Iodine species and the endocrine system
T2 - Thyroid hormone levels in adult Danio rerio and developing Xenopus laevis
AU - Simon, R.
AU - Tietge, J. E.
AU - Michalke, B.
AU - Degitz, S.
AU - Schramm, K. W.
PY - 2002
Y1 - 2002
N2 - Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS). This method enables quantification of iodide, T4 and T3, as well as reverse T3 (rT3) and the synthetic precursors of TH, monoiodotyrosine (MIT), and diiodotyrosine (DIT) in a single injection. In this work, the LC-ICP-MS approach was used to analyze whole-body homogenates of adult male and female zebrafish (Danio rerio) and tadpoles of the African clawed frog (Xenopus laevis) at two different developmental stages (NF58 and 61) according to Nieuwkoop and Faber. The data demonstrate that the LC-ICP-MS method was successful at measuring I-, MIT, DIT, T4, T3, and rT3 in these two species. Furthermore, the method also detected five additional iodinated compounds which are currently unidentified.
AB - Recently a new approach for the analysis of iodinated organic species in human serum has been developed using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS). This method enables quantification of iodide, T4 and T3, as well as reverse T3 (rT3) and the synthetic precursors of TH, monoiodotyrosine (MIT), and diiodotyrosine (DIT) in a single injection. In this work, the LC-ICP-MS approach was used to analyze whole-body homogenates of adult male and female zebrafish (Danio rerio) and tadpoles of the African clawed frog (Xenopus laevis) at two different developmental stages (NF58 and 61) according to Nieuwkoop and Faber. The data demonstrate that the LC-ICP-MS method was successful at measuring I-, MIT, DIT, T4, T3, and rT3 in these two species. Furthermore, the method also detected five additional iodinated compounds which are currently unidentified.
KW - Iodine species
KW - Thyroid
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=0036170971&partnerID=8YFLogxK
U2 - 10.1007/s00216-001-1211-9
DO - 10.1007/s00216-001-1211-9
M3 - Article
C2 - 11939537
AN - SCOPUS:0036170971
SN - 0937-0633
VL - 372
SP - 481
EP - 485
JO - Fresenius' Journal of Analytical Chemistry
JF - Fresenius' Journal of Analytical Chemistry
IS - 3
ER -