Investigation of two deoxygenated haemoglobin-Haptoglobin complexes by Mössbauer spectroscopy

A. Alfsen; D. Bade; U. van Bürck; H. Eicher; S. Formanek; G. M. Kalvius; F. Lavialle; A. Mayer; F. Parak; J. Tejada; U. F. Thomanek

doi:10.1007/BF00535698

Investigation of two deoxygenated haemoglobin-Haptoglobin complexes by Mössbauer spectroscopy

A. Alfsen, D. Bade, U. van Bürck, H. Eicher, S. Formanek, G. M. Kalvius, F. Lavialle, A. Mayer, F. Parak, J. Tejada, U. F. Thomanek

Chair of Biomedical Physics

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Abstract

Haemoglobin Haptoglobin complexes formed when [Hp⁺]/[Hb] = 1/1 and [Hp]/[Hb] = 2/1 were investigated by ⁵⁷Fe Mössbauer spectroscopy. Both samples gave a spectrum consisting of a single quadrupole doublet. The temperature dependence of the quadrupole splitting was also identical for both samples. This proves that in both samples the nearest neighbour environment of the iron atom must be the same. A comparison with earlier investigations on myoglobin and haemoglobin indicates that the electronic structure of iron in the HbHp-complexes is similar to that in myoglobin.

Original language	English
Pages (from-to)	229-238
Number of pages	10
Journal	Biophysics of Structure and Mechanism
Volume	3
Issue number	3-4
DOIs	https://doi.org/10.1007/BF00535698
State	Published - Sep 1977

Keywords

Haemoglobin
Haptoglobin
Mössbauer spectroscopy

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10.1007/BF00535698

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title = "Investigation of two deoxygenated haemoglobin-Haptoglobin complexes by M{\"o}ssbauer spectroscopy",

abstract = "Haemoglobin Haptoglobin complexes formed when [Hp+]/[Hb] = 1/1 and [Hp]/[Hb] = 2/1 were investigated by 57Fe M{\"o}ssbauer spectroscopy. Both samples gave a spectrum consisting of a single quadrupole doublet. The temperature dependence of the quadrupole splitting was also identical for both samples. This proves that in both samples the nearest neighbour environment of the iron atom must be the same. A comparison with earlier investigations on myoglobin and haemoglobin indicates that the electronic structure of iron in the HbHp-complexes is similar to that in myoglobin.",

keywords = "Haemoglobin, Haptoglobin, M{\"o}ssbauer spectroscopy",

author = "A. Alfsen and D. Bade and \{van B{\"u}rck\}, U. and H. Eicher and S. Formanek and Kalvius, \{G. M.\} and F. Lavialle and A. Mayer and F. Parak and J. Tejada and Thomanek, \{U. F.\}",

year = "1977",

month = sep,

doi = "10.1007/BF00535698",

language = "English",

volume = "3",

pages = "229--238",

journal = "Biophysics of Structure and Mechanism",

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TY - JOUR

T1 - Investigation of two deoxygenated haemoglobin-Haptoglobin complexes by Mössbauer spectroscopy

AU - Alfsen, A.

AU - Bade, D.

AU - van Bürck, U.

AU - Eicher, H.

AU - Formanek, S.

AU - Kalvius, G. M.

AU - Lavialle, F.

AU - Mayer, A.

AU - Parak, F.

AU - Tejada, J.

AU - Thomanek, U. F.

PY - 1977/9

Y1 - 1977/9

N2 - Haemoglobin Haptoglobin complexes formed when [Hp+]/[Hb] = 1/1 and [Hp]/[Hb] = 2/1 were investigated by 57Fe Mössbauer spectroscopy. Both samples gave a spectrum consisting of a single quadrupole doublet. The temperature dependence of the quadrupole splitting was also identical for both samples. This proves that in both samples the nearest neighbour environment of the iron atom must be the same. A comparison with earlier investigations on myoglobin and haemoglobin indicates that the electronic structure of iron in the HbHp-complexes is similar to that in myoglobin.

AB - Haemoglobin Haptoglobin complexes formed when [Hp+]/[Hb] = 1/1 and [Hp]/[Hb] = 2/1 were investigated by 57Fe Mössbauer spectroscopy. Both samples gave a spectrum consisting of a single quadrupole doublet. The temperature dependence of the quadrupole splitting was also identical for both samples. This proves that in both samples the nearest neighbour environment of the iron atom must be the same. A comparison with earlier investigations on myoglobin and haemoglobin indicates that the electronic structure of iron in the HbHp-complexes is similar to that in myoglobin.

KW - Haemoglobin

KW - Haptoglobin

KW - Mössbauer spectroscopy

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U2 - 10.1007/BF00535698

DO - 10.1007/BF00535698

M3 - Article

C2 - 901911

AN - SCOPUS:0017759672

SN - 0340-1057

VL - 3

SP - 229

EP - 238

JO - Biophysics of Structure and Mechanism

JF - Biophysics of Structure and Mechanism

IS - 3-4

ER -

Investigation of two deoxygenated haemoglobin-Haptoglobin complexes by Mössbauer spectroscopy

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Keywords

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