Abstract
As an alternative to optical flow cytometry, magnetic flow cytometry has emerged in recent years for single cell analysis with sheath-less flow conditions. Instead of measuring scattered light, magnetic fields of magneticallylabeled cells are recorded and analyzed. To determine microfluidic opportunities to manipulate cell trajectories for in-situ cell focusing we use mechanical guiding structures and magnetophoretic forces. A 3D-microfluidic particle simulation has been implemented that revealed parameters ensuring the crucial balance between hydrodynamic drag and magnetophoretic forces. Experimental measurements verified the 3D particle tracking simulation.
| Original language | English |
|---|---|
| Pages (from-to) | 353-355 |
| Number of pages | 3 |
| Journal | Current Directions in Biomedical Engineering |
| Volume | 5 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1 Sep 2019 |
| Externally published | Yes |
Keywords
- Magnetic flow cytometry
- cell focusing
- magnetophoresis
- microfluidics
- particle focusing