Investigation of a tetracycline-regulated phage display system

G. Zahn, A. Skerra, W. Höhne

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

A new vector (pGZ1) was developed for bacterial phage display of antibody fragments using a transcriptional regulation element with tight control. The tet(p/o)-based phasmid exhibits fully suppressed scFv background synthesis in the absence of inducer and is independent of glucose as a catabolite repressor. The vector is shown to be a useful alternative to commonly used lac(p/o)-regulated systems.

Original languageEnglish
Pages (from-to)1031-1034
Number of pages4
JournalProtein Engineering
Volume12
Issue number12
DOIs
StatePublished - 1999

Keywords

  • Phagemid
  • Single-chain Fv
  • Tetracycline promoter
  • pHen

Fingerprint

Dive into the research topics of 'Investigation of a tetracycline-regulated phage display system'. Together they form a unique fingerprint.

Cite this