Abstract
A new vector (pGZ1) was developed for bacterial phage display of antibody fragments using a transcriptional regulation element with tight control. The tet(p/o)-based phasmid exhibits fully suppressed scFv background synthesis in the absence of inducer and is independent of glucose as a catabolite repressor. The vector is shown to be a useful alternative to commonly used lac(p/o)-regulated systems.
Original language | English |
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Pages (from-to) | 1031-1034 |
Number of pages | 4 |
Journal | Protein Engineering |
Volume | 12 |
Issue number | 12 |
DOIs | |
State | Published - 1999 |
Keywords
- Phagemid
- Single-chain Fv
- Tetracycline promoter
- pHen