TY - JOUR
T1 - Investigating Microglia in Health and Disease
T2 - Challenges and Opportunities
AU - Miron, Veronique E.
AU - Priller, Josef
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/9
Y1 - 2020/9
N2 - Microglia are tissue-resident macrophages implicated in central nervous system (CNS) development, homeostasis, and response to injury. Recent advances in transcriptomics, multiplex protein expression analysis, and experimental depletion of microglia have cemented their importance. However, it is still unclear which models are best suited to investigate microglia and explore their function in human disease. Here, we discuss issues regarding off-targeting during experimental manipulation, and differences and similarities between human and rodent microglia. With new developments in transgenic lines and human–rodent chimeras, we anticipate that in coming years, a clearer picture of microglia function in health and disease will emerge.
AB - Microglia are tissue-resident macrophages implicated in central nervous system (CNS) development, homeostasis, and response to injury. Recent advances in transcriptomics, multiplex protein expression analysis, and experimental depletion of microglia have cemented their importance. However, it is still unclear which models are best suited to investigate microglia and explore their function in human disease. Here, we discuss issues regarding off-targeting during experimental manipulation, and differences and similarities between human and rodent microglia. With new developments in transgenic lines and human–rodent chimeras, we anticipate that in coming years, a clearer picture of microglia function in health and disease will emerge.
UR - http://www.scopus.com/inward/record.url?scp=85088809102&partnerID=8YFLogxK
U2 - 10.1016/j.it.2020.07.002
DO - 10.1016/j.it.2020.07.002
M3 - Review article
C2 - 32736967
AN - SCOPUS:85088809102
SN - 1471-4906
VL - 41
SP - 785
EP - 793
JO - Trends in Immunology
JF - Trends in Immunology
IS - 9
ER -
