Introduction of the transposon Tn919 into Lactobacillus curvatus Lc2-c

Hermann J. Knauf, Rudi F. Vogel, Walter P. Hammes

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The conjugative transposon Tn919 was introduced into the plasmid cured strain Lactobacillus curvatus Lc 2-c by means of filter mating. Enterococcus faecalis GF590 was used as donor. The transfer frequency was 2 × 10-7/recipient. Random insertion was proved by Southern hybridization. Segregation after 10 passages without selective pressure could not be detected. The transposition site remained unchanged and no subsequent second-site transposition occured.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalFEMS Microbiology Letters
Volume65
Issue number1-2
DOIs
StatePublished - Nov 1989
Externally publishedYes

Keywords

  • Chromosomal insertion
  • Lactobacillus curvatus
  • Transposition

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