Intravital optoacoustic and ultrasound bio-microscopy reveal radiation-inhibited skull angiogenesis

Héctor Estrada, Johannes Rebling, Wolfgang Sievert, Daniela Hladik, Urs Hofmann, Sven Gottschalk, Soile Tapio, Gabriele Multhoff, Daniel Razansky

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Angiogenesis is critical in bone development and growth. Dense, large-scale, and multi-layered vascular networks formed by thin-walled sinusoidal vessels perfuse the plate bones and play an important role in bone repair. Yet, the intricate functional morphology of skull microvasculature remains poorly understood as it is difficult to visualize using existing intravital microscopy techniques. Here we introduced an intravital, fully-transcranial imaging approach based on hybrid optoacoustic and ultrasound bio-microscopy for large-scale observations and quantitative analysis of the vascular morphology, angiogenesis, vessel remodeling, and subsurface roughness in murine skulls. Our approach revealed radiation-inhibited angiogenesis in the skull bone. We also observed previously undocumented sinusoidal vascular networks spanning the entire skullcap, thus opening new vistas for studying the complex interactions between calvarial, pial, and cortical vascular systems.

Original languageEnglish
Article number115251
JournalBone
Volume133
DOIs
StatePublished - Apr 2020

Keywords

  • Bone angiogenesis
  • Image segmentation
  • Optoacoustic microscopy
  • Quantitative vasculature analysis
  • Radiation
  • Skull vasculature
  • Ultrasound microscopy

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