TY - GEN
T1 - Intravital hybrid optical-optoacoustic microscopy based on fiber-Bragg interferometry
AU - Shnaiderman, Rami
AU - Wissmeyer, Georg
AU - Seeger, Markus
AU - Estrada, Hector
AU - Ntziachristos, Vasilis
N1 - Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
PY - 2018
Y1 - 2018
N2 - Optoacoustic microscopy (OAM) has enabled high-resolution, label-free imaging of tissues at depths not achievable with purely optical microscopy. However, widespread implementation of OAM into existing epi-illumination microscopy setups is often constrained by the performance and size of the commonly used piezoelectric ultrasound detectors. In this work, we introduce a novel acoustic detector based on a π-phase-shifted fiber Bragg grating (π-FBG) interferometer embedded inside an ellipsoidal acoustic cavity. The cavity enables seamless integration of epi-illumination OAM into existing microscopy setups by decoupling the acoustic and optical paths between the microscope objective and the sample. The cavity also acts as an acoustic condenser, boosting the sensitivity of the π-FBG and enabling cost effective CW-laser interrogation technique. We characterize the sensor's sensitivity and bandwidth and demonstrate hybrid OAM and second-harmonic imaging of phantoms and mouse tissue in vivo.
AB - Optoacoustic microscopy (OAM) has enabled high-resolution, label-free imaging of tissues at depths not achievable with purely optical microscopy. However, widespread implementation of OAM into existing epi-illumination microscopy setups is often constrained by the performance and size of the commonly used piezoelectric ultrasound detectors. In this work, we introduce a novel acoustic detector based on a π-phase-shifted fiber Bragg grating (π-FBG) interferometer embedded inside an ellipsoidal acoustic cavity. The cavity enables seamless integration of epi-illumination OAM into existing microscopy setups by decoupling the acoustic and optical paths between the microscope objective and the sample. The cavity also acts as an acoustic condenser, boosting the sensitivity of the π-FBG and enabling cost effective CW-laser interrogation technique. We characterize the sensor's sensitivity and bandwidth and demonstrate hybrid OAM and second-harmonic imaging of phantoms and mouse tissue in vivo.
KW - Medical and biological imaging
KW - fiber Bragg- Grating
KW - fiber detectors
KW - nonlinear microscopy
KW - optoacoustic microscopy
UR - http://www.scopus.com/inward/record.url?scp=85047353242&partnerID=8YFLogxK
U2 - 10.1117/12.2290281
DO - 10.1117/12.2290281
M3 - Conference contribution
AN - SCOPUS:85047353242
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photons Plus Ultrasound
A2 - Wang, Lihong V.
A2 - Oraevsky, Alexander A.
PB - SPIE
T2 - Photons Plus Ultrasound: Imaging and Sensing 2018
Y2 - 28 January 2018 through 1 February 2018
ER -