Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes

H. Welzl; T. R. Tölle; J. P. Huston

doi:10.1016/0165-0270(85)90039-1

Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes

H. Welzl, T. R. Tölle, J. P. Huston

Heinrich-Heine-University

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A method is described which allows intracranial injection of drug solutions through glass micropipettes (tip diameter 15 μm) in the freely moving rat by use of air pressure. Compared with conventional injection methods through steel cannulae this method has the advantages of (1) minimal destruction of brain tissue, (2) precise injection of small volumes of solutions (1 nl to several microliters), (3) simultaneous recording of local EEG activity, and (4) usage of multipipette assemblies.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Journal of Neuroscience Methods
Volume	13
Issue number	1
DOIs	https://doi.org/10.1016/0165-0270(85)90039-1
State	Published - Mar 1985
Externally published	Yes

Keywords

chemical stimulation
intracranial injection
pressure injection
rat

Access to Document

10.1016/0165-0270(85)90039-1

Cite this

@article{3e10cb68b26a4cf1b47e81a50da1622a,

title = "Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes",

abstract = "A method is described which allows intracranial injection of drug solutions through glass micropipettes (tip diameter 15 μm) in the freely moving rat by use of air pressure. Compared with conventional injection methods through steel cannulae this method has the advantages of (1) minimal destruction of brain tissue, (2) precise injection of small volumes of solutions (1 nl to several microliters), (3) simultaneous recording of local EEG activity, and (4) usage of multipipette assemblies.",

keywords = "chemical stimulation, intracranial injection, pressure injection, rat",

author = "H. Welzl and T{\"o}lle, {T. R.} and Huston, {J. P.}",

note = "Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft 306/4-1.",

year = "1985",

month = mar,

doi = "10.1016/0165-0270(85)90039-1",

language = "English",

volume = "13",

pages = "1--8",

journal = "Journal of Neuroscience Methods",

issn = "0165-0270",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes

AU - Welzl, H.

AU - Tölle, T. R.

AU - Huston, J. P.

N1 - Funding Information: This work was supported by the Deutsche Forschungsgemeinschaft 306/4-1.

PY - 1985/3

Y1 - 1985/3

N2 - A method is described which allows intracranial injection of drug solutions through glass micropipettes (tip diameter 15 μm) in the freely moving rat by use of air pressure. Compared with conventional injection methods through steel cannulae this method has the advantages of (1) minimal destruction of brain tissue, (2) precise injection of small volumes of solutions (1 nl to several microliters), (3) simultaneous recording of local EEG activity, and (4) usage of multipipette assemblies.

AB - A method is described which allows intracranial injection of drug solutions through glass micropipettes (tip diameter 15 μm) in the freely moving rat by use of air pressure. Compared with conventional injection methods through steel cannulae this method has the advantages of (1) minimal destruction of brain tissue, (2) precise injection of small volumes of solutions (1 nl to several microliters), (3) simultaneous recording of local EEG activity, and (4) usage of multipipette assemblies.

KW - chemical stimulation

KW - intracranial injection

KW - pressure injection

KW - rat

UR - http://www.scopus.com/inward/record.url?scp=0021907168&partnerID=8YFLogxK

U2 - 10.1016/0165-0270(85)90039-1

DO - 10.1016/0165-0270(85)90039-1

M3 - Article

C2 - 3990341

AN - SCOPUS:0021907168

SN - 0165-0270

VL - 13

SP - 1

EP - 8

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 1

ER -

Intracranial application of substances in the unrestrained, awake rat by pressure injection through glass micropipettes

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this