TY - JOUR
T1 - Intracellular routing of human amyloid protein precursor
T2 - Axonal delivery followed by transport to the dendrites
AU - Simons, M.
AU - Ikonen, E.
AU - Tienari, P. J.
AU - Cid‐Arregui, A.
AU - Mönning, U.
AU - Beyreuther, K.
AU - Dotti, C. G.
PY - 1995/5/1
Y1 - 1995/5/1
N2 - A characteristic neuropathological feature of Alzheimer's disease is the cerebral deposition of amyloid plaques. These deposits contain b̃A4 amyloid peptide, a cleavage product of the transmembrane protein amyloid protein precursor (APP). Despite numerous studies on the processing of the different APP isoforms in non‐neuronal cells, little is known about its sorting and transport in neurons cf the central nervous system (CNS). To analyze this question we expressed in cultured rat hippocampal neurons the human APP 695, tagged at its N‐terminus with the myc epitope, using the Semliki forest virus (SFV) expression system. APP was first delivered from the cell body to the axon and later appeared also in the dendrites. Inhibition of protein synthesis at the time of axonal expression did not block the late appearance of the protein in the dendrites. An antibody directed against the myc tag, bound to the cell surface at 4°C at the time of axonal APP expression, could be chased to the dendritic domain after subsequent incubation at 37°C. These results suggest that the newly synthesized APP, after initial axonal delivery, may be transported to the dendrites by a transcytotic mechanism. The routing of APP in polarized neurons is different from that of polarized epithelial cells, in which the protein is delivered basolaterally, arguing for neuronal specific sorting and processing mechanisms. © 1995 Wiley‐Liss, Inc.
AB - A characteristic neuropathological feature of Alzheimer's disease is the cerebral deposition of amyloid plaques. These deposits contain b̃A4 amyloid peptide, a cleavage product of the transmembrane protein amyloid protein precursor (APP). Despite numerous studies on the processing of the different APP isoforms in non‐neuronal cells, little is known about its sorting and transport in neurons cf the central nervous system (CNS). To analyze this question we expressed in cultured rat hippocampal neurons the human APP 695, tagged at its N‐terminus with the myc epitope, using the Semliki forest virus (SFV) expression system. APP was first delivered from the cell body to the axon and later appeared also in the dendrites. Inhibition of protein synthesis at the time of axonal expression did not block the late appearance of the protein in the dendrites. An antibody directed against the myc tag, bound to the cell surface at 4°C at the time of axonal APP expression, could be chased to the dendritic domain after subsequent incubation at 37°C. These results suggest that the newly synthesized APP, after initial axonal delivery, may be transported to the dendrites by a transcytotic mechanism. The routing of APP in polarized neurons is different from that of polarized epithelial cells, in which the protein is delivered basolaterally, arguing for neuronal specific sorting and processing mechanisms. © 1995 Wiley‐Liss, Inc.
KW - amyloid protein precursor
KW - cultured CNS neurons
KW - neuron polarity
KW - transcytosis
KW - viral expression vector
UR - http://www.scopus.com/inward/record.url?scp=0028988649&partnerID=8YFLogxK
U2 - 10.1002/jnr.490410114
DO - 10.1002/jnr.490410114
M3 - Article
C2 - 7674373
AN - SCOPUS:0028988649
SN - 0360-4012
VL - 41
SP - 121
EP - 128
JO - Journal of Neuroscience Research
JF - Journal of Neuroscience Research
IS - 1
ER -