Intestinimonas butyriciproducens gen. nov., sp. nov., a butyrate-producing bacterium from the mouse intestine

Karoline Kläring, Laura Hanske, Nam Bui, Cédric Charrier, Michael Blaut, Dirk Haller, Caroline M. Plugge, Thomas Clavel

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

A Gram-positive, spore-forming, non-motile, strictly anaerobic rod-shaped bacterium was isolated from the caecal content of a TNFdeltaARE mouse. The isolate, referred to as strain SRB-521-5-IT, was originally cultured on a reduced agar medium containing yeast extract, rumen fluid and lactic acid as main energy and carbon sources. Phylogenetic analysis of partial 16S rRNA genes revealed that the species most closely related to strain SRB-521-5-IT were Flavonifractor plautii and Pseudoflavonifractor capillosus (<95 % sequence similarity; 1436 bp). In contrast to F. plautii and P. capillosus, strain SRB-521-5-IT contained a substantial amount of C18: 0 dimethylacetal. Additional major fatty acids were C14: 0 methyl ester, C16: 0 dimethylacetal and C18: 0 aldehyde. Strain SRB-521-5-IT differed in its enzyme profile from F. plautii and P. capillosus by being positive for dextrin, maltotriose, turanose, DL-lactic acid and D-lactic acid methyl ester but negative for D-fructose. In reduced Wilkins-Chalgren-Anaerobe broth, strain SRB-521-5-IT produced approximately 8 mM butyrate and 4 mM acetate. In contrast to F. plautii, the strain did not metabolize flavonoids. It showed intermediate resistance towards the antibiotics ciprofloxacin, colistin and tetracycline. Based on genotypic and phenotypic characteristics, we propose the name Intestinimonas butyriciproducens gen. nov., sp. nov. to accommodate strain SRB-521-5-IT (= DSM 26588T = CCUG 63529T) as the type strain.

Original languageEnglish
Pages (from-to)4606-4612
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume63
Issue numberPART 12
DOIs
StatePublished - Dec 2013

Fingerprint

Dive into the research topics of 'Intestinimonas butyriciproducens gen. nov., sp. nov., a butyrate-producing bacterium from the mouse intestine'. Together they form a unique fingerprint.

Cite this