TY - JOUR
T1 - Interaction of ultraviolet-B-rich and ultraviolet-A-rich radiation in ketoprofen-induced photohemolysis
AU - Eberlein-König, Bernadette
AU - Rueff, F.
AU - Przybilla, B.
PY - 1996
Y1 - 1996
N2 - Interactions between different wavelengths can cause inhibition or enhancement of various biological reactions. We evaluated in vitro the effect of UVB-rich irradiation on UVA-induced phototoxicity. Suspensions of human erythrocytes were incubated with ketoprofen, a phototoxic nonsteroidal anti-inflammatory drug. These samples were exposed to 0, 20, 40, 80, 160 or 320 mJ/cm2 UVB followed by irradiation with 0 or 6 J/cm2 UVA. Photo-induced hemolysis was calculated as a percentage of complete hemolysis. Ketoprofen-dependent hemolysis due to UVA alone was 63% (median). UVB did not induce ketoprofen-dependent photohemolysis at doses <80 mJ/cm2. Exposure to UVB at all doses enhanced significantly (P<0.01) UVA-induced ketoprofen-dependent hemolysis in samples from 9 of 15 donors. However, with erythrocytes from the other 6 donors, 20 or 40 mJ/cm2 UVB reduced the median of UVA-induced photohemolysis by 69% (P<0.05) or 47% (not significant), respectively. Co-incubation of samples with ascorbic acid resulted in a profound inhibition of ketoprofen-dependent photohemolysis. These results indicate that the effect of low UVB doses on UVA-induced phototoxicity depends on individual, yet unknown characteristics of the target cells.
AB - Interactions between different wavelengths can cause inhibition or enhancement of various biological reactions. We evaluated in vitro the effect of UVB-rich irradiation on UVA-induced phototoxicity. Suspensions of human erythrocytes were incubated with ketoprofen, a phototoxic nonsteroidal anti-inflammatory drug. These samples were exposed to 0, 20, 40, 80, 160 or 320 mJ/cm2 UVB followed by irradiation with 0 or 6 J/cm2 UVA. Photo-induced hemolysis was calculated as a percentage of complete hemolysis. Ketoprofen-dependent hemolysis due to UVA alone was 63% (median). UVB did not induce ketoprofen-dependent photohemolysis at doses <80 mJ/cm2. Exposure to UVB at all doses enhanced significantly (P<0.01) UVA-induced ketoprofen-dependent hemolysis in samples from 9 of 15 donors. However, with erythrocytes from the other 6 donors, 20 or 40 mJ/cm2 UVB reduced the median of UVA-induced photohemolysis by 69% (P<0.05) or 47% (not significant), respectively. Co-incubation of samples with ascorbic acid resulted in a profound inhibition of ketoprofen-dependent photohemolysis. These results indicate that the effect of low UVB doses on UVA-induced phototoxicity depends on individual, yet unknown characteristics of the target cells.
KW - UVA and UVB interaction
KW - ascorbic acid
KW - ketoprofen
KW - photohemolysis
KW - phototoxicity
UR - http://www.scopus.com/inward/record.url?scp=0029826698&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0781.1996.tb00186.x
DO - 10.1111/j.1600-0781.1996.tb00186.x
M3 - Article
C2 - 8956360
AN - SCOPUS:0029826698
SN - 0905-4383
VL - 12
SP - 109
EP - 113
JO - Photodermatology Photoimmunology and Photomedicine
JF - Photodermatology Photoimmunology and Photomedicine
IS - 3
ER -