Interaction of respiratory and photosynthetic electron transport, and evidence for membrane‐bound pyridine‐nucleotide dehydrogenases in Anabaena variabilis

Erwin Stürzl; Siegfried Scherer; Peter Böger

doi:10.1111/j.1399-3054.1984.tb04915.x

Interaction of respiratory and photosynthetic electron transport, and evidence for membrane‐bound pyridine‐nucleotide dehydrogenases in Anabaena variabilis

Erwin Stürzl, Siegfried Scherer, Peter Böger

Universitat Konstanz

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Membranes isolated from vegetative cells of Anabaena variabilis (ATCC 29413) oxidine NADPH and NADH in a cyanide‐sensitive reaction, NADPH being the better donor. In addition, both act as reductants for photosystem I in the light and in the presence of DCMU. An NADH‐regenerating system has been introduced experimentally, functioning as donor system for photosystem I of Anabaena membranes. This NADH dehydrogenation is strictly light‐dependent, sensitive to DBMIB, establishes a transhydrogenase system with NADP⁺ was hydrogen acceptor and competes with oxygen. Kinetic analysis of oxygen uptake with reference to nucleotide concentration exhibited different K_m‐ and V_max‐values for NADPH and NADH in the light and in the dark.

Original language	English
Pages (from-to)	479-483
Number of pages	5
Journal	Physiologia Plantarum
Volume	60
Issue number	4
DOIs	https://doi.org/10.1111/j.1399-3054.1984.tb04915.x
State	Published - Apr 1984
Externally published	Yes

Keywords

Blue‐green alga
NAD(P)H
NADH/NADP transhydrogenase system
cyanobacteria
electron donors
ferredoxin‐NADP oxidoreductase

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10.1111/j.1399-3054.1984.tb04915.x

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title = "Interaction of respiratory and photosynthetic electron transport, and evidence for membrane‐bound pyridine‐nucleotide dehydrogenases in Anabaena variabilis",

abstract = "Membranes isolated from vegetative cells of Anabaena variabilis (ATCC 29413) oxidine NADPH and NADH in a cyanide‐sensitive reaction, NADPH being the better donor. In addition, both act as reductants for photosystem I in the light and in the presence of DCMU. An NADH‐regenerating system has been introduced experimentally, functioning as donor system for photosystem I of Anabaena membranes. This NADH dehydrogenation is strictly light‐dependent, sensitive to DBMIB, establishes a transhydrogenase system with NADP+ was hydrogen acceptor and competes with oxygen. Kinetic analysis of oxygen uptake with reference to nucleotide concentration exhibited different Km‐ and Vmax‐values for NADPH and NADH in the light and in the dark.",

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author = "Erwin St{\"u}rzl and Siegfried Scherer and Peter B{\"o}ger",

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AU - Stürzl, Erwin

AU - Scherer, Siegfried

AU - Böger, Peter

PY - 1984/4

Y1 - 1984/4

N2 - Membranes isolated from vegetative cells of Anabaena variabilis (ATCC 29413) oxidine NADPH and NADH in a cyanide‐sensitive reaction, NADPH being the better donor. In addition, both act as reductants for photosystem I in the light and in the presence of DCMU. An NADH‐regenerating system has been introduced experimentally, functioning as donor system for photosystem I of Anabaena membranes. This NADH dehydrogenation is strictly light‐dependent, sensitive to DBMIB, establishes a transhydrogenase system with NADP+ was hydrogen acceptor and competes with oxygen. Kinetic analysis of oxygen uptake with reference to nucleotide concentration exhibited different Km‐ and Vmax‐values for NADPH and NADH in the light and in the dark.

AB - Membranes isolated from vegetative cells of Anabaena variabilis (ATCC 29413) oxidine NADPH and NADH in a cyanide‐sensitive reaction, NADPH being the better donor. In addition, both act as reductants for photosystem I in the light and in the presence of DCMU. An NADH‐regenerating system has been introduced experimentally, functioning as donor system for photosystem I of Anabaena membranes. This NADH dehydrogenation is strictly light‐dependent, sensitive to DBMIB, establishes a transhydrogenase system with NADP+ was hydrogen acceptor and competes with oxygen. Kinetic analysis of oxygen uptake with reference to nucleotide concentration exhibited different Km‐ and Vmax‐values for NADPH and NADH in the light and in the dark.

KW - Blue‐green alga

KW - NAD(P)H

KW - NADH/NADP transhydrogenase system

KW - cyanobacteria

KW - electron donors

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Interaction of respiratory and photosynthetic electron transport, and evidence for membrane‐bound pyridine‐nucleotide dehydrogenases in Anabaena variabilis

Abstract

Keywords

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