Insights into the role of phytohormones regulating pAtNIP5;1 activity and boron transport in Arabidopsis thaliana

D. Gómez-Soto, S. Galván, E. Rosales, P. Bienert, I. Abreu, I. Bonilla, L. Bolaños, M. Reguera

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Aiming to counteract B deficiency impacts, plants have developed different strategies in order to reach an optimal growth in soils with limited B availability. These include B transport mechanisms that involves a facilitated transport, via channel proteins, and a high-affinity active transport driven by borate transporters. The AtNIP5;1 channel protein is a member of Major Intrinsic Protein family which facilitates B influx into the roots under low B supply. In order to explore the phytohormone-dependent regulation of AtNIP5;1, the effects of abscisic acid (ABA), ethylene, auxins and cytokinins on the activity of AtNIP5;1 promoter were evaluated using the reporter line pNIP5;1-GUS. The results show that ABA treatment increased pAtNIP5;1 activity. Besides, a larger B uptake was found following ABA treatment under B deficiency suggesting a role of ABA inducing B uptake. The ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) caused an induction of AtNIP5;1 expression although did not correlate with higher B concentrations nor with an improvement in root growth. On the contrary, auxins and cytokinins caused slight changes in pAtNIP5;1 induction. Altogether, these results show a regulatory role of phytohormones in AtNIP5;1 promoter what may affect B transport. The herein provided information may contribute to better understand the regulation of B transport in plants towards minimizing B deficiency impacts on agriculture.

Original languageEnglish
Article number110198
JournalPlant Science
Volume287
DOIs
StatePublished - Oct 2019
Externally publishedYes

Keywords

  • ABA
  • Aquoporins
  • AtNIP5, 1
  • Auxin
  • Boron deficiency
  • Boron transport
  • Cytokinin
  • Ethylene
  • Phytohormones
  • Transcriptional regulation

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