Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

H. Erdinc Besikcioglu; Ümmügülsüm Yurteri; Enkhtsetseg Munkhbaatar; Linhan Ye; Fangfang Zhang; Alessandra Moretti; Carmen Mota Reyes; Candan Özoğul; Helmut Friess; Güralp O. Ceyhan; Rouzanna Istvanffy; Ihsan Ekin Demir

doi:10.1016/j.xpro.2021.100935

Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

H. Erdinc Besikcioglu, Ümmügülsüm Yurteri, Enkhtsetseg Munkhbaatar, Linhan Ye, Fangfang Zhang, Alessandra Moretti, Carmen Mota Reyes, Candan Özoğul, Helmut Friess, Güralp O. Ceyhan, Rouzanna Istvanffy, Ihsan Ekin Demir

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras^+/LSLG12D Trp53^{fl/f l}p48^+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons. For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).

Original language	English
Article number	100935
Journal	STAR Protocols
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.xpro.2021.100935
State	Published - 17 Dec 2021

Keywords

Cancer
Cell culture
Cell Differentiation
Cell isolation
Model Organisms
Neuroscience
Organoids
Stem Cells

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.xpro.2021.100935

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title = "Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer",

abstract = "Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons. For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).",

keywords = "Cancer, Cell culture, Cell Differentiation, Cell isolation, Model Organisms, Neuroscience, Organoids, Stem Cells",

author = "Besikcioglu, {H. Erdinc} and {\"U}mm{\"u}g{\"u}ls{\"u}m Yurteri and Enkhtsetseg Munkhbaatar and Linhan Ye and Fangfang Zhang and Alessandra Moretti and {Mota Reyes}, Carmen and Candan {\"O}zoğul and Helmut Friess and Ceyhan, {G{\"u}ralp O.} and Rouzanna Istvanffy and Demir, {Ihsan Ekin}",

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year = "2021",

month = dec,

day = "17",

doi = "10.1016/j.xpro.2021.100935",

language = "English",

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T1 - Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

AU - Besikcioglu, H. Erdinc

AU - Yurteri, Ümmügülsüm

AU - Munkhbaatar, Enkhtsetseg

AU - Ye, Linhan

AU - Zhang, Fangfang

AU - Moretti, Alessandra

AU - Mota Reyes, Carmen

AU - Özoğul, Candan

AU - Friess, Helmut

AU - Ceyhan, Güralp O.

AU - Istvanffy, Rouzanna

AU - Demir, Ihsan Ekin

PY - 2021/12/17

Y1 - 2021/12/17

N2 - Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons. For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).

AB - Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons. For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).

KW - Cancer

KW - Cell culture

KW - Cell Differentiation

KW - Cell isolation

KW - Model Organisms

KW - Neuroscience

KW - Organoids

KW - Stem Cells

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U2 - 10.1016/j.xpro.2021.100935

DO - 10.1016/j.xpro.2021.100935

M3 - Article

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AN - SCOPUS:85119173072

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 4

M1 - 100935

ER -

Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

Abstract

Keywords

UN SDGs

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