Innervated mouse pancreas organoids as an ex vivo model to study pancreatic neuropathy in pancreatic cancer

H. Erdinc Besikcioglu, Ümmügülsüm Yurteri, Enkhtsetseg Munkhbaatar, Linhan Ye, Fangfang Zhang, Alessandra Moretti, Carmen Mota Reyes, Candan Özoğul, Helmut Friess, Güralp O. Ceyhan, Rouzanna Istvanffy, Ihsan Ekin Demir

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Pancreatic cancer is characterized by bi-directional interactions between pancreatic cancer cells and stromal cells including neural cells. The absence of neural cells in pancreatic organoids limits the investigation of cell- cell interaction and tumor innervation. This protocol describes how to generate innervated wild type (WT) and Kras+/LSLG12D Trp53fl/f lp48+/Cre (KPC) murine pancreatic organoids. To specifically investigate neurogenesis, organoids are co-cultured with iPSCs-derived neural crest cells, while co-culture with dorsal root ganglia explants is used for comparing organoids with mature neurons. For complete details on the use and execution of this protocol, please refer to Huch et al. (2013), Boj et al. (2015), and Demir et al. (2014).

Original languageEnglish
Article number100935
JournalSTAR Protocols
Volume2
Issue number4
DOIs
StatePublished - 17 Dec 2021
Externally publishedYes

Keywords

  • Cancer
  • Cell Differentiation
  • Cell culture
  • Cell isolation
  • Model Organisms
  • Neuroscience
  • Organoids
  • Stem Cells

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