TY - JOUR
T1 - Inhibiton of growth by 2,3,7,8-terachlorodibezo-p-dioxin in 5l rat hepatoma cells is associated with the presence of ah receptor
AU - Göttlicher, Martin
AU - Cikryt, Peter
AU - Wiebel, Friedrich J.
N1 - Funding Information:
The excellent technical assistance of F Kiefer is gratefully acknowledged The work was partially supported by the Deutsche Forschungsgemeinschaft (fellowship to M.G.) and by the Bundesministerium fQr Forschung und Technologie (07DIX10 0, PC.)
PY - 1990/12
Y1 - 1990/12
N2 - The role of the Ah receptor in mediating the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was investigated in 5L rat hepatoma cells containing TCDD-inducible cytochrome P450IA1 activity and in variants Lacking cytochrome P4SOIA1 and Ah receptor. TCDD inhibited growth of the wild-type 5L cells, but not of the Ah receptor deficient variants. The two strong Ah receptor ligands 3,3' ,4,4'-tetrachlorobiphenyl (3,3' ,4,4'-TCB) and benz [α]anthracene (BA) exerted toxic effects in 5L cells that resembled those of TCDD. The poor Ah receptor ligand 2,2' ,4,4'-tetrachloroblphenyl was not toxic in 5L cells. The concentrations of TCDD, 3,3' ,4,4'-TCB or BA required for the toxic response were similar to those that elicited P450IA1 induction. The present results suggest strongly that interac tion with the Ah receptor is a necessary link in the chain of events leading to toxic effects in 5L cells upon exposure to TCDD.
AB - The role of the Ah receptor in mediating the toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) was investigated in 5L rat hepatoma cells containing TCDD-inducible cytochrome P450IA1 activity and in variants Lacking cytochrome P4SOIA1 and Ah receptor. TCDD inhibited growth of the wild-type 5L cells, but not of the Ah receptor deficient variants. The two strong Ah receptor ligands 3,3' ,4,4'-tetrachlorobiphenyl (3,3' ,4,4'-TCB) and benz [α]anthracene (BA) exerted toxic effects in 5L cells that resembled those of TCDD. The poor Ah receptor ligand 2,2' ,4,4'-tetrachloroblphenyl was not toxic in 5L cells. The concentrations of TCDD, 3,3' ,4,4'-TCB or BA required for the toxic response were similar to those that elicited P450IA1 induction. The present results suggest strongly that interac tion with the Ah receptor is a necessary link in the chain of events leading to toxic effects in 5L cells upon exposure to TCDD.
UR - http://www.scopus.com/inward/record.url?scp=0025676130&partnerID=8YFLogxK
U2 - 10.1093/carcin/11.12.2205
DO - 10.1093/carcin/11.12.2205
M3 - Article
C2 - 2176138
AN - SCOPUS:0025676130
SN - 0143-3334
VL - 11
SP - 2205
EP - 2210
JO - Carcinogenesis
JF - Carcinogenesis
IS - 12
ER -