Inhibition of woodchuck hepatitis virus gene expression in primary hepatocytes by siRNA enhances the cellular gene expression

Zhongji Meng; Song Qiu; Xiaoyong Zhang; Jun Wu; Thomas Schreiter; Yang Xu; Dongliang Yang; Michael Roggendorf; Jörg Schlaak; Mengji Lu

doi:10.1016/j.virol.2008.11.012

Inhibition of woodchuck hepatitis virus gene expression in primary hepatocytes by siRNA enhances the cellular gene expression

Zhongji Meng, Song Qiu, Xiaoyong Zhang, Jun Wu, Thomas Schreiter, Yang Xu, Dongliang Yang, Michael Roggendorf, Jörg Schlaak, Mengji Lu

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Small interfering RNA (siRNA) has been shown to be active to inhibit the hepatitis B virus gene expression and replication in transient and stable transfection systems. Here in primary hepatocytes prepared from naturally woodchuck hepatitis virus (WHV)-infected woodchucks, four siRNAs targeting the WHV preS1, S, C, and X region led to a depletion of WHV transcripts and replicative intermediates with different kinetics and a decreased production of viral particles. Two siRNAs targeting WHV S and X region had the highest efficacy to deplete 70% of WHV transcripts and replicative intermediates. In addition, siRNA-mediated suppression of WHV enhanced the expression of cellular genes like MxA and MHC I. Specific siRNAs are able to inhibit the hepadnaviral replication and enhance the expression of cellular genes relevant for antiviral actions. Thus, siRNAs might be useful as novel antiviral agents for the treatment of chronic HBV infection.

Original language	English
Pages (from-to)	88-96
Number of pages	9
Journal	Virology
Volume	384
Issue number	1
DOIs	https://doi.org/10.1016/j.virol.2008.11.012
State	Published - 5 Feb 2009
Externally published	Yes

Keywords

Hepadnavirus
IFN-stimulated genes
ISGs
RNA interference
RNAi
Woodchuck

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.virol.2008.11.012

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title = "Inhibition of woodchuck hepatitis virus gene expression in primary hepatocytes by siRNA enhances the cellular gene expression",

abstract = "Small interfering RNA (siRNA) has been shown to be active to inhibit the hepatitis B virus gene expression and replication in transient and stable transfection systems. Here in primary hepatocytes prepared from naturally woodchuck hepatitis virus (WHV)-infected woodchucks, four siRNAs targeting the WHV preS1, S, C, and X region led to a depletion of WHV transcripts and replicative intermediates with different kinetics and a decreased production of viral particles. Two siRNAs targeting WHV S and X region had the highest efficacy to deplete 70% of WHV transcripts and replicative intermediates. In addition, siRNA-mediated suppression of WHV enhanced the expression of cellular genes like MxA and MHC I. Specific siRNAs are able to inhibit the hepadnaviral replication and enhance the expression of cellular genes relevant for antiviral actions. Thus, siRNAs might be useful as novel antiviral agents for the treatment of chronic HBV infection.",

keywords = "Hepadnavirus, IFN-stimulated genes, ISGs, RNA interference, RNAi, Woodchuck",

author = "Zhongji Meng and Song Qiu and Xiaoyong Zhang and Jun Wu and Thomas Schreiter and Yang Xu and Dongliang Yang and Michael Roggendorf and J{\"o}rg Schlaak and Mengji Lu",

note = "Funding Information: This work is supported by grants of the Deutsche Forschungsgemeinschaft (Lu 669/2-1, GRK1045/1, and Lu 669/5-1), the Federal Ministry of Education and Research (GU0207), the National Natural Science Foundation of China (30271170, 30571646) and the National High Technology Research and Development Program of China (2006AA02Z128).",

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doi = "10.1016/j.virol.2008.11.012",

language = "English",

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journal = "Virology",

issn = "0042-6822",

publisher = "Academic Press Inc.",

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T1 - Inhibition of woodchuck hepatitis virus gene expression in primary hepatocytes by siRNA enhances the cellular gene expression

AU - Meng, Zhongji

AU - Qiu, Song

AU - Zhang, Xiaoyong

AU - Wu, Jun

AU - Schreiter, Thomas

AU - Xu, Yang

AU - Yang, Dongliang

AU - Roggendorf, Michael

AU - Schlaak, Jörg

AU - Lu, Mengji

N1 - Funding Information: This work is supported by grants of the Deutsche Forschungsgemeinschaft (Lu 669/2-1, GRK1045/1, and Lu 669/5-1), the Federal Ministry of Education and Research (GU0207), the National Natural Science Foundation of China (30271170, 30571646) and the National High Technology Research and Development Program of China (2006AA02Z128).

PY - 2009/2/5

Y1 - 2009/2/5

N2 - Small interfering RNA (siRNA) has been shown to be active to inhibit the hepatitis B virus gene expression and replication in transient and stable transfection systems. Here in primary hepatocytes prepared from naturally woodchuck hepatitis virus (WHV)-infected woodchucks, four siRNAs targeting the WHV preS1, S, C, and X region led to a depletion of WHV transcripts and replicative intermediates with different kinetics and a decreased production of viral particles. Two siRNAs targeting WHV S and X region had the highest efficacy to deplete 70% of WHV transcripts and replicative intermediates. In addition, siRNA-mediated suppression of WHV enhanced the expression of cellular genes like MxA and MHC I. Specific siRNAs are able to inhibit the hepadnaviral replication and enhance the expression of cellular genes relevant for antiviral actions. Thus, siRNAs might be useful as novel antiviral agents for the treatment of chronic HBV infection.

AB - Small interfering RNA (siRNA) has been shown to be active to inhibit the hepatitis B virus gene expression and replication in transient and stable transfection systems. Here in primary hepatocytes prepared from naturally woodchuck hepatitis virus (WHV)-infected woodchucks, four siRNAs targeting the WHV preS1, S, C, and X region led to a depletion of WHV transcripts and replicative intermediates with different kinetics and a decreased production of viral particles. Two siRNAs targeting WHV S and X region had the highest efficacy to deplete 70% of WHV transcripts and replicative intermediates. In addition, siRNA-mediated suppression of WHV enhanced the expression of cellular genes like MxA and MHC I. Specific siRNAs are able to inhibit the hepadnaviral replication and enhance the expression of cellular genes relevant for antiviral actions. Thus, siRNAs might be useful as novel antiviral agents for the treatment of chronic HBV infection.

KW - Hepadnavirus

KW - IFN-stimulated genes

KW - ISGs

KW - RNA interference

KW - RNAi

KW - Woodchuck

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Inhibition of woodchuck hepatitis virus gene expression in primary hepatocytes by siRNA enhances the cellular gene expression

Abstract

Keywords

UN SDGs

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