TY - JOUR
T1 - Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes
AU - Fehrenbach, E.
AU - Niess, A. M.
AU - Passek, F.
AU - Sorichter, S.
AU - Schwirtz, A.
AU - Berg, A.
AU - Dickhuth, H. H.
AU - Northoff, H.
N1 - Funding Information:
This investigation was supported by a grant from the Bundesinstitut für Sportwissenschaft (Köln, Germany, VF 0407/01/21/97). We would like to thank the athletes who volunteered to participate in the study.
PY - 2003
Y1 - 2003
N2 - Haem-oxygenase-1 (HO-1) is an antioxidant stress protein that is mainly induced by reactive oxygen species, inflammatory cytokines and hyperthermia. We assessed the influence of different types of exercise on HO-1 expression in leukocytes of the peripheral blood in three groups of male participants: a short exhaustive run above the lactate steady state (n =15), eccentric exercise (n =12) and an intensive endurance run (halfmarathon, n = 12). Blood samples were taken at rest and up to 24 h after exercise. Blood lactate concentration after exercise was 9.0 ±2.1, 3.8 ±1.6 and 5.1 ±2.2 mmol. 1-1 (mean ±s) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). Creatine kinase concentration was highest 24 h after exercise: 133 ±91, 231 ±139 and 289 ±221 U. 1-1 for the exhaustive run, eccentric exercise and halfmarathon groups, respectively (P < 0.05). The maximal increase in leukocyte counts after exercise was 11.5 ±19.2, 6.2 ±1.4 and 14.7 ±2.1 x 109. l 71. There was no change in HO-1 as a result of the short exhaustive run or the eccentric exercise, whereas the half-marathon had a significant stimulatory effect on HO- 1-expression in lymphocytes, monocytes and granulocytes (P<0.001) using flow cytometry analyses. In conclusion, eccentric exercise alone or short-term heavy exercise are not sufficient to stimulate the antioxidative stress protein HO-1 in peripheral leukocytes.
AB - Haem-oxygenase-1 (HO-1) is an antioxidant stress protein that is mainly induced by reactive oxygen species, inflammatory cytokines and hyperthermia. We assessed the influence of different types of exercise on HO-1 expression in leukocytes of the peripheral blood in three groups of male participants: a short exhaustive run above the lactate steady state (n =15), eccentric exercise (n =12) and an intensive endurance run (halfmarathon, n = 12). Blood samples were taken at rest and up to 24 h after exercise. Blood lactate concentration after exercise was 9.0 ±2.1, 3.8 ±1.6 and 5.1 ±2.2 mmol. 1-1 (mean ±s) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). Creatine kinase concentration was highest 24 h after exercise: 133 ±91, 231 ±139 and 289 ±221 U. 1-1 for the exhaustive run, eccentric exercise and halfmarathon groups, respectively (P < 0.05). The maximal increase in leukocyte counts after exercise was 11.5 ±19.2, 6.2 ±1.4 and 14.7 ±2.1 x 109. l 71. There was no change in HO-1 as a result of the short exhaustive run or the eccentric exercise, whereas the half-marathon had a significant stimulatory effect on HO- 1-expression in lymphocytes, monocytes and granulocytes (P<0.001) using flow cytometry analyses. In conclusion, eccentric exercise alone or short-term heavy exercise are not sufficient to stimulate the antioxidative stress protein HO-1 in peripheral leukocytes.
KW - Concentric
KW - Eccentric
KW - Endurance
KW - Haem oxygenase-1
KW - Heat shock protein
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=0037500297&partnerID=8YFLogxK
U2 - 10.1080/0264041031000071164
DO - 10.1080/0264041031000071164
M3 - Article
C2 - 12800860
AN - SCOPUS:0037500297
SN - 0264-0414
VL - 21
SP - 383
EP - 389
JO - Journal of Sports Sciences
JF - Journal of Sports Sciences
IS - 5
ER -