Influence of different types of exercise on the expression of haem oxygenase-1 in leukocytes

E. Fehrenbach, A. M. Niess, F. Passek, S. Sorichter, A. Schwirtz, A. Berg, H. H. Dickhuth, H. Northoff

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25 Scopus citations

Abstract

Haem-oxygenase-1 (HO-1) is an antioxidant stress protein that is mainly induced by reactive oxygen species, inflammatory cytokines and hyperthermia. We assessed the influence of different types of exercise on HO-1 expression in leukocytes of the peripheral blood in three groups of male participants: a short exhaustive run above the lactate steady state (n =15), eccentric exercise (n =12) and an intensive endurance run (halfmarathon, n = 12). Blood samples were taken at rest and up to 24 h after exercise. Blood lactate concentration after exercise was 9.0 ±2.1, 3.8 ±1.6 and 5.1 ±2.2 mmol. 1-1 (mean ±s) for the exhaustive run, eccentric exercise and half-marathon groups, respectively (P < 0.05). Creatine kinase concentration was highest 24 h after exercise: 133 ±91, 231 ±139 and 289 ±221 U. 1-1 for the exhaustive run, eccentric exercise and halfmarathon groups, respectively (P < 0.05). The maximal increase in leukocyte counts after exercise was 11.5 ±19.2, 6.2 ±1.4 and 14.7 ±2.1 x 109. l 71. There was no change in HO-1 as a result of the short exhaustive run or the eccentric exercise, whereas the half-marathon had a significant stimulatory effect on HO- 1-expression in lymphocytes, monocytes and granulocytes (P<0.001) using flow cytometry analyses. In conclusion, eccentric exercise alone or short-term heavy exercise are not sufficient to stimulate the antioxidative stress protein HO-1 in peripheral leukocytes.

Original languageEnglish
Pages (from-to)383-389
Number of pages7
JournalJournal of Sports Sciences
Volume21
Issue number5
DOIs
StatePublished - 2003
Externally publishedYes

Keywords

  • Concentric
  • Eccentric
  • Endurance
  • Haem oxygenase-1
  • Heat shock protein
  • Oxidative stress

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