Influence of collagenase on tip links in hair cells of the chick basilar papilla

J. O. Pickles; J. Brix; G. A. Manley

doi:10.1016/0378-5955(90)90040-V

Influence of collagenase on tip links in hair cells of the chick basilar papilla

J. O. Pickles, J. Brix, G. A. Manley

Life Sciences

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9 Scopus citations

Abstract

Chick basilar papillae were incubated in collagenase, using concentrations previously employed for the isolation of viable hair cells. When assessed by scanning electron microscopy, the hair bundles had a normal conformation, with no loss of tip links compared with control incubations. The results suggest that collagenase does not destroy the integrity of structures on the apical surface of hair cells and that tip links are not composed of collagen. The results are in agreement with the hypothesis that tip links are involved in mechanotransduction.

Original language	English
Pages (from-to)	139-143
Number of pages	5
Journal	Hearing Research
Volume	50
Issue number	1-2
DOIs	https://doi.org/10.1016/0378-5955(90)90040-V
State	Published - Dec 1990

Keywords

Chick
Collagenase
Hyaluronidase
Stereocilia
Tip link
Transduction

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10.1016/0378-5955(90)90040-V

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title = "Influence of collagenase on tip links in hair cells of the chick basilar papilla",

abstract = "Chick basilar papillae were incubated in collagenase, using concentrations previously employed for the isolation of viable hair cells. When assessed by scanning electron microscopy, the hair bundles had a normal conformation, with no loss of tip links compared with control incubations. The results suggest that collagenase does not destroy the integrity of structures on the apical surface of hair cells and that tip links are not composed of collagen. The results are in agreement with the hypothesis that tip links are involved in mechanotransduction.",

keywords = "Chick, Collagenase, Hyaluronidase, Stereocilia, Tip link, Transduction",

author = "Pickles, {J. O.} and J. Brix and Manley, {G. A.}",

note = "Funding Information: This work was supported by the Medical Research Council of the U.K., the Deutsche For-schungsgemeinschaftw ithin the programme of the SFB 204 {\textquoteleft}Gehor{\textquoteright}, the Commission of the European Community, The Hearing and Speech Trust, and the Australian Research Council.",

year = "1990",

month = dec,

doi = "10.1016/0378-5955(90)90040-V",

language = "English",

volume = "50",

pages = "139--143",

journal = "Hearing Research",

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TY - JOUR

T1 - Influence of collagenase on tip links in hair cells of the chick basilar papilla

AU - Pickles, J. O.

AU - Brix, J.

AU - Manley, G. A.

N1 - Funding Information: This work was supported by the Medical Research Council of the U.K., the Deutsche For-schungsgemeinschaftw ithin the programme of the SFB 204 ‘Gehor’, the Commission of the European Community, The Hearing and Speech Trust, and the Australian Research Council.

PY - 1990/12

Y1 - 1990/12

N2 - Chick basilar papillae were incubated in collagenase, using concentrations previously employed for the isolation of viable hair cells. When assessed by scanning electron microscopy, the hair bundles had a normal conformation, with no loss of tip links compared with control incubations. The results suggest that collagenase does not destroy the integrity of structures on the apical surface of hair cells and that tip links are not composed of collagen. The results are in agreement with the hypothesis that tip links are involved in mechanotransduction.

AB - Chick basilar papillae were incubated in collagenase, using concentrations previously employed for the isolation of viable hair cells. When assessed by scanning electron microscopy, the hair bundles had a normal conformation, with no loss of tip links compared with control incubations. The results suggest that collagenase does not destroy the integrity of structures on the apical surface of hair cells and that tip links are not composed of collagen. The results are in agreement with the hypothesis that tip links are involved in mechanotransduction.

KW - Chick

KW - Collagenase

KW - Hyaluronidase

KW - Stereocilia

KW - Tip link

KW - Transduction

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U2 - 10.1016/0378-5955(90)90040-V

DO - 10.1016/0378-5955(90)90040-V

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C2 - 1963885

AN - SCOPUS:0025614368

SN - 0378-5955

VL - 50

SP - 139

EP - 143

JO - Hearing Research

JF - Hearing Research

IS - 1-2

ER -

Influence of collagenase on tip links in hair cells of the chick basilar papilla

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Keywords

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