TY - JOUR
T1 - Influence of changes in microbial cell membrane composition on isotopic fractionation of nitrate during denitrification
AU - Wunderlich, Anja
AU - Heipieper, Hermann J.
AU - Elsner, Martin
AU - Einsiedl, Florian
N1 - Publisher Copyright:
© The Authors, published by EDP Sciences, 2019.
PY - 2019/6/7
Y1 - 2019/6/7
N2 - Pure cultures of Thauera aromatica were stressed by the addition of the toxic solvents 1-octanol and 4-chlorophenol causing adaptive modifications in their cell-membrane fatty acid composition. At the same time, we investigated the isotopic fractionation in δ15N-NO3- during denitrification. We observed a change in the degree of saturation (DoS) of the bacteria as a reaction to solvent stress and a higher degree of saturation was directly correlated to the concentration of solvents in the cell membrane. The enrichment factor ϵ15N-NO3- during denitrification showed a linear dependency to the DoS as well as to the membrane solvent concentration. Denitrifying bacteria thus may express less negative ϵ15N-NO3- when they are exposed to environmental stress that changes their cell membrane composition.
AB - Pure cultures of Thauera aromatica were stressed by the addition of the toxic solvents 1-octanol and 4-chlorophenol causing adaptive modifications in their cell-membrane fatty acid composition. At the same time, we investigated the isotopic fractionation in δ15N-NO3- during denitrification. We observed a change in the degree of saturation (DoS) of the bacteria as a reaction to solvent stress and a higher degree of saturation was directly correlated to the concentration of solvents in the cell membrane. The enrichment factor ϵ15N-NO3- during denitrification showed a linear dependency to the DoS as well as to the membrane solvent concentration. Denitrifying bacteria thus may express less negative ϵ15N-NO3- when they are exposed to environmental stress that changes their cell membrane composition.
UR - http://www.scopus.com/inward/record.url?scp=85067308302&partnerID=8YFLogxK
U2 - 10.1051/e3sconf/20199801051
DO - 10.1051/e3sconf/20199801051
M3 - Conference article
AN - SCOPUS:85067308302
SN - 2555-0403
VL - 98
JO - E3S Web of Conferences
JF - E3S Web of Conferences
M1 - 01051
T2 - 16th International Symposium on Water-Rock Interaction, WRI 2019 and 13th International Symposium on Applied Isotope Geochemistry, 1st IAGC International Conference
Y2 - 21 July 2019 through 26 July 2019
ER -