Induction of cell polarization and migration by a gradient of nanoscale variations in adhesive ligand spacing

Marco Arnold, Vera C. Hirschfeld-Warneken, Theobald Lohmüller, Patrick Heil, Jacques Blümmel, Elisabetta A. Cavalcanti-Adam, Mónica López-García, Paul Walther, Horst Kessler, Benjamin Geiger, Joachim P. Spatz

Research output: Contribution to journalArticlepeer-review

264 Scopus citations

Abstract

Cell interactions with adhesive surfaces play a vital role in the regulation of cell proliferation, viability, and differentiation, and affect multiple biological processes. Since cell adhesion depends mainly on the nature and density of the adhesive ligand molecules, spatial molecular patterning, which enables the modulation of adhesion receptor clustering, might affect both the structural and the signaling activities of the adhesive interaction. We herein show that cells plated on surfaces that present a molecularly defined spacing gradient of an integrin RGD ligand can sense small but consistent differences in adhesive ligand spacing of about 1 nm across the cell diameter, which is approximately 61 μm when the spacing includes 70 nm. Consequently, these positional cues induce cell polarization and initiate cell migration and signaling. We propose that differential positional clustering of the integrin transmembrane receptors is used by cells for exploring and interpreting their environment, at high spatial sensitivity.

Original languageEnglish
Pages (from-to)2063-2069
Number of pages7
JournalNano Letters
Volume8
Issue number7
DOIs
StatePublished - Jul 2008

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