Induction of a T helper cell response against the tumor-associated antigen HER2 using monocyte-derived dendritic cells

J. Metzger, N. Nicklisch, P. Kufer, C. Peschel, P. B. Luppa, H. Bernhard

Research output: Contribution to journalArticlepeer-review

Abstract

Tumor-reactive CD4+ T helper (Th) cells play a critical role in antitumor immunity, due to their ability to induce CD8+ T cell-mediated cytotoxic activity and humoral response. This study focuses on the in vitro generation and expansion of Th cells specific for the tumor-associated antigen 'human epidermal growth factor receptor-2' (HER2). A protocol for efficient HER2 presentation was developed using autologous monocyte-derived dendritic cells (DC) as antigen presenting cells (APC) and purified HER2 protein as antigen source. Our data suggest that DC pulsed with recombinant protein of the extracellular domain (ECD) of HER2 (ECD/HER2) induce an ECD/HER2-specific Th cell response. This finding may facilitate the development of immunotherapy regimens without requiring defined immunogenic epitopes of the antigen.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalCytotechnology
Volume38
Issue number1-3
DOIs
StatePublished - 2002

Keywords

  • Adoptive T cell transfer
  • Breast cancer
  • Dendritic cells
  • HER2
  • Th cells
  • Tumor-associated antigen

Fingerprint

Dive into the research topics of 'Induction of a T helper cell response against the tumor-associated antigen HER2 using monocyte-derived dendritic cells'. Together they form a unique fingerprint.

Cite this