Inducing microscopic thermal lesions for the dissection of functional cell networks on a chip

Philipp Rinklin, Dzmitry Afanasenkau, Simone Wiegand, Andreas Offenhäusser, Bernhard Wolfrum

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

We present a versatile chip-based method to inflict microscopic lesions on cellular networks or tissue models. Our approach relies on resistive heating of microstructured conductors to impose highly localized thermal stress on specific regions of a cell network. We show that networks can be precisely dissected into individual subnetworks using a microwire crossbar array. To this end, we pattern a network of actively beating cardiomyocyte-like cells into smaller subunits by inflicting thermal damage along selected wires of the array. We then investigate the activity and functional connectivity of the individual subnetworks using a Ca2+ imaging-based signal propagation analysis. Our results demonstrate the efficient separation of functional activity between individual subnetworks on a microscopic level. We believe that the presented technique may become a powerful tool for investigating lesion and regeneration models in cellular networks.

Original languageEnglish
Pages (from-to)237-243
Number of pages7
JournalLab on a Chip
Volume15
Issue number1
DOIs
StatePublished - 7 Jan 2015
Externally publishedYes

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