Inducible gene deletion in astroglia and radial glia - A valuable tool for functional and lineage analysis

Tetsuji Mori, Kohichi Tanaka, Annalisa Buffo, Wolfgang Wurst, Ralf Kühn, Magdalena Gotz

Research output: Contribution to journalArticlepeer-review

303 Scopus citations

Abstract

Astrocytes are thought to play a variety of key roles in the adult brain, such as their participation in synaptic transmission, in wound healing upon brain injury, and adult neurogenesis. However, to elucidate these functions in vivo has been difficult because of the lack of astrocyte-specific gene targeting. Here we show that the inducible form of Cre (CreERT2) expressed in the locus of the astrocyte-specific glutamate transporter (GLAST) allows precisely timed gene deletion in adult astrocytes as well as radial glial cells at earlier developmental stages. Moreover, postnatal and adult neurogenesis can be targeted at different stages with high efficiency as it originates from astroglial cells. Taken together, this mouse line will allow dissecting the molecular pathways regulating the diverse functions of astrocytes as precursors, support cells, repair cells, and cells involved in neuronal information processing.

Original languageEnglish
Pages (from-to)21-34
Number of pages14
JournalGLIA
Volume54
Issue number1
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • Adult neurogenesis
  • Astrocyte
  • CreERT2
  • GLAST
  • Inducible cre activity
  • Radial glia

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