Indole-3-acetic Acid and p-Hydroxyacetophenone Driven Ethylene Formation from 1-Aminocyclopropane-1-carboxylic Acid Catalyzed by Horseradish Peroxidase

Wolfgang F. Osswald; Wolfgang Schütz; Erich F. Elstner

doi:10.1016/S0176-1617(89)80019-7

Indole-3-acetic Acid and p-Hydroxyacetophenone Driven Ethylene Formation from 1-Aminocyclopropane-1-carboxylic Acid Catalyzed by Horseradish Peroxidase

Wolfgang F. Osswald, Wolfgang Schütz, Erich F. Elstner

Technical University of Munich

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

An in vitro system is described in which ethylene formation from the amino acid, 1-aminocyclopropane- 1-carboxylic acid (ACC), is catalyzed by horseradish peroxidase (HRP) in the presence of Mn²⁺ and p-hydroxyacetophenone (pHAP) as cofactors. The monophenol can be substituted for by indole-3-acetic acid or indole-3-methanol. Oxidative ACC breakdown is accelerated at acidic pH whereas ethylene formation in the presence of IAA is stimulated by pHAP with a pH optimum at 7.8. ACC oxidation is inhibited by propylgallate and ascorbic acid by 100%, whereas OH scavengers such as mannitol or formate have no effect.

Original language	English
Pages (from-to)	510-513
Number of pages	4
Journal	Journal of Plant Physiology
Volume	134
Issue number	4
DOIs	https://doi.org/10.1016/S0176-1617(89)80019-7
State	Published - 1989

Keywords

1-aminocyclopropane-1-carboxylic acid
2,4-dichlorophenol
ACC
ACC-oxidation
Cat
DCP
HRP
Horseradish Peroxidase (HRP)
IAA
IM
SOD
catalase
ethylene formation
horseradish peroxidase
indole-3-acetic acid
indole-3-acetic acid
indole-3-methanol
p-hydroxyacetophenone
p-hydroxyacetophenone
pHAP
superoxide dismutase

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10.1016/S0176-1617(89)80019-7

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@article{6a81962461814439a2412c35599e1700,

title = "Indole-3-acetic Acid and p-Hydroxyacetophenone Driven Ethylene Formation from 1-Aminocyclopropane-1-carboxylic Acid Catalyzed by Horseradish Peroxidase",

abstract = "An in vitro system is described in which ethylene formation from the amino acid, 1-aminocyclopropane- 1-carboxylic acid (ACC), is catalyzed by horseradish peroxidase (HRP) in the presence of Mn2+ and p-hydroxyacetophenone (pHAP) as cofactors. The monophenol can be substituted for by indole-3-acetic acid or indole-3-methanol. Oxidative ACC breakdown is accelerated at acidic pH whereas ethylene formation in the presence of IAA is stimulated by pHAP with a pH optimum at 7.8. ACC oxidation is inhibited by propylgallate and ascorbic acid by 100%, whereas OH scavengers such as mannitol or formate have no effect.",

keywords = "1-aminocyclopropane-1-carboxylic acid, 2,4-dichlorophenol, ACC, ACC-oxidation, Cat, DCP, HRP, Horseradish Peroxidase (HRP), IAA, IM, SOD, catalase, ethylene formation, horseradish peroxidase, indole-3-acetic acid, indole-3-acetic acid, indole-3-methanol, p-hydroxyacetophenone, p-hydroxyacetophenone, pHAP, superoxide dismutase",

author = "Osswald, {Wolfgang F.} and Wolfgang Sch{\"u}tz and Elstner, {Erich F.}",

year = "1989",

doi = "10.1016/S0176-1617(89)80019-7",

language = "English",

volume = "134",

pages = "510--513",

journal = "Journal of Plant Physiology",

issn = "0176-1617",

publisher = "Elsevier GmbH",

number = "4",

}

TY - JOUR

T1 - Indole-3-acetic Acid and p-Hydroxyacetophenone Driven Ethylene Formation from 1-Aminocyclopropane-1-carboxylic Acid Catalyzed by Horseradish Peroxidase

AU - Osswald, Wolfgang F.

AU - Schütz, Wolfgang

AU - Elstner, Erich F.

PY - 1989

Y1 - 1989

N2 - An in vitro system is described in which ethylene formation from the amino acid, 1-aminocyclopropane- 1-carboxylic acid (ACC), is catalyzed by horseradish peroxidase (HRP) in the presence of Mn2+ and p-hydroxyacetophenone (pHAP) as cofactors. The monophenol can be substituted for by indole-3-acetic acid or indole-3-methanol. Oxidative ACC breakdown is accelerated at acidic pH whereas ethylene formation in the presence of IAA is stimulated by pHAP with a pH optimum at 7.8. ACC oxidation is inhibited by propylgallate and ascorbic acid by 100%, whereas OH scavengers such as mannitol or formate have no effect.

AB - An in vitro system is described in which ethylene formation from the amino acid, 1-aminocyclopropane- 1-carboxylic acid (ACC), is catalyzed by horseradish peroxidase (HRP) in the presence of Mn2+ and p-hydroxyacetophenone (pHAP) as cofactors. The monophenol can be substituted for by indole-3-acetic acid or indole-3-methanol. Oxidative ACC breakdown is accelerated at acidic pH whereas ethylene formation in the presence of IAA is stimulated by pHAP with a pH optimum at 7.8. ACC oxidation is inhibited by propylgallate and ascorbic acid by 100%, whereas OH scavengers such as mannitol or formate have no effect.

KW - 1-aminocyclopropane-1-carboxylic acid

KW - 2,4-dichlorophenol

KW - ACC

KW - ACC-oxidation

KW - Cat

KW - DCP

KW - HRP

KW - Horseradish Peroxidase (HRP)

KW - IAA

KW - IM

KW - SOD

KW - catalase

KW - ethylene formation

KW - horseradish peroxidase

KW - indole-3-acetic acid

KW - indole-3-methanol

KW - p-hydroxyacetophenone

KW - pHAP

KW - superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=85013793374&partnerID=8YFLogxK

U2 - 10.1016/S0176-1617(89)80019-7

DO - 10.1016/S0176-1617(89)80019-7

M3 - Article

AN - SCOPUS:85013793374

SN - 0176-1617

VL - 134

SP - 510

EP - 513

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 4

ER -

Indole-3-acetic Acid and p-Hydroxyacetophenone Driven Ethylene Formation from 1-Aminocyclopropane-1-carboxylic Acid Catalyzed by Horseradish Peroxidase

Abstract

Keywords

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