Abstract
A new enrichment approach is described to improve the sensitivity of the asymmetrical flow field-flow fractionation, with and without ICPMS online coupling for elemental detection. For the slot outlet technique, a part of the laminar carrier stream is removed through an additional pump. This allowed an enrichment of the colloidal particles in the separation channel up to a factor of 14. Additional improvements of the separation efficiency permitted us to separate colloids with differences in their molecular masses of only 2 kDa. Different polymer standards, proteins, and synthesized tracer colloids, as well as real samples (wastewater, liquid manure, serums) were used to assess the performance of the new technique.
| Original language | English |
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| Pages (from-to) | 6664-6669 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 78 |
| Issue number | 18 |
| DOIs | |
| State | Published - 15 Sep 2006 |