@inproceedings{212b2e3c9eb94dc18ea25bc540211933,
title = "In-vivo red fluorescent protein tomography",
abstract = "We report on a novel mutli-spectral tomographic method that allows the 3D visualization of fluorescence protein activity in small animals. The method makes use of a novel spectral inversion scheme that integrates three-dimensional image reconstruction and auto-fluorescence correction that works seamlessly in the steep absorption transition from visible to near-infrared and yields an accurate whole-body redshifted FP tomography platform for small animals. We demonstrate the method imaging mCherry fluorescent protein labeled glioma tumors in animal brains operating with twoorders of magnitude better sensitivity compared to visualizing GFP. The method developed offers a new visualization dimension for functional genomics and proteomics, stem cell research and related modern biology trends in need of in-vivo longitudinal visualization of cellular and sub-cellular activity.",
keywords = "Autofluorescence, Fluorescent proteins, Molecular imaging, Multispectral, Tomography",
author = "Deliolanis, {N. C.} and T. Wurdinger and L. Pike and B. Tannous and V. Ntziachristos",
year = "2009",
doi = "10.1007/978-3-642-03879-2_76",
language = "English",
isbn = "9783642038785",
series = "IFMBE Proceedings",
publisher = "Springer Verlag",
number = "2",
pages = "270--273",
booktitle = "World Congress on Medical Physics and Biomedical Engineering",
edition = "2",
note = "World Congress on Medical Physics and Biomedical Engineering: Diagnostic Imaging ; Conference date: 07-09-2009 Through 12-09-2009",
}