In vivo molecular imaging of chemokine receptor CXCR4 expression in patients with advanced multiple myeloma

Kathrin Philipp-Abbrederis, Ken Herrmann, Stefan Knop, Margret Schottelius, Matthias Eiber, Katharina Lückerath, Elke Pietschmann, Stefan Habringer, Carlos Gerngroß, Katharina Franke, Martina Rudelius, Andreas Schirbel, Constantin Lapa, Kristina Schwamborn, Sabine Steidle, Elena Hartmann, Andreas Rosenwald, Saskia Kropf, Ambros J. Beer, Christian PeschelHermann Einsele, Andreas K. Buck, Markus Schwaiger, Katharina Götze, Hans Jürgen Wester, Ulrich Keller

Research output: Contribution to journalArticlepeer-review

189 Scopus citations

Abstract

CXCR4 is a G-protein-coupled receptor that mediates recruitment of blood cells toward its ligand SDF-1. In cancer, high CXCR4 expression is frequently associated with tumor dissemination and poor prognosis. We evaluated the novel CXCR4 probe [68Ga]Pentixafor for in vivo mapping of CXCR4 expression density in mice xenografted with human CXCR4-positive MM cell lines and patients with advanced MM by means of positron emission tomography (PET). [68Ga]Pentixafor PET provided images with excellent specificity and contrast. In 10 of 14 patients with advanced MM [68Ga]Pentixafor PET/CT scans revealed MM manifestations, whereas only nine of 14 standard [18F]fluorodeoxyglucose PET/CT scans were rated visually positive. Assessment of blood counts and standard CD34+ flow cytometry did not reveal significant blood count changes associated with tracer application. Based on these highly encouraging data on clinical PET imaging of CXCR4 expression in a cohort of MM patients, we conclude that [68Ga]Pentixafor PET opens a broad field for clinical investigations on CXCR4 expression and for CXCR4-directed therapeutic approaches in MM and other diseases.

Original languageEnglish
Pages (from-to)477-487
Number of pages11
JournalEMBO Molecular Medicine
Volume7
Issue number4
DOIs
StatePublished - 1 Apr 2015

Keywords

  • CXCR4
  • Chemokine receptor
  • Multiple myeloma
  • Positron emission tomography
  • in vivo imaging

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