In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography

Nikolaos C. Deliolanis; Joshua Dunham; Thomas Wurdinger; Jose Luiz Figueiredo; Bakhos A. Tannous; Vasilis Ntziachristos

doi:10.1117/1.3149854

In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography

Nikolaos C. Deliolanis, Joshua Dunham, Thomas Wurdinger, Jose Luiz Figueiredo, Bakhos A. Tannous, Vasilis Ntziachristos

Chair of Biological Imaging

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for the heterogeneous distribution of photons in tissue. In-vivo imaging is then showcased on mouse lung and brain tumors cross-validated by x-ray microcomputed tomography and histology.

Original language	English
Article number	030509
Journal	Journal of Biomedical Optics
Volume	14
Issue number	3
DOIs	https://doi.org/10.1117/1.3149854
State	Published - 2009

Keywords

cancer
fluorescence
fluorescent proteins
in vivo
molecular imaging
tomography
tumor

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1117/1.3149854

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title = "In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography",

abstract = "We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for the heterogeneous distribution of photons in tissue. In-vivo imaging is then showcased on mouse lung and brain tumors cross-validated by x-ray microcomputed tomography and histology.",

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AU - Deliolanis, Nikolaos C.

AU - Dunham, Joshua

AU - Wurdinger, Thomas

AU - Figueiredo, Jose Luiz

AU - Tannous, Bakhos A.

AU - Ntziachristos, Vasilis

PY - 2009

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AB - We interrogate the ability of free-space fluorescence tomography to image small animals in vivo using charge-coupled device (CCD) camera measurements over 360-deg noncontact projections. We demonstrate the performance of normalized dual-wavelength measurements that are essential for in-vivo use, as they account for the heterogeneous distribution of photons in tissue. In-vivo imaging is then showcased on mouse lung and brain tumors cross-validated by x-ray microcomputed tomography and histology.

KW - cancer

KW - fluorescence

KW - fluorescent proteins

KW - in vivo

KW - molecular imaging

KW - tomography

KW - tumor

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ER -

In-vivo imaging of murine tumors using complete-angle projection fluorescence molecular tomography

Abstract

Keywords

UN SDGs

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