In vivo imaging of mitochondria in intact zebrafish larvae

Dominik Paquet, Gabriela Plucińska, Thomas Misgeld

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

8 Scopus citations

Abstract

Visualizing neuronal mitochondria in a living, intact mammalian organism is a challenge that can be overcome in zebrafish larvae, which are highly accessible for optical imaging and genetic manipulation. Here, we detail an approach to visualize neuronal mitochondria in sensory Rohon-Beard axons, which allows quantitatively measuring mitochondrial shape, dynamics, and transport in vivo. This provides a useful assay for basic studies exploring the behavior of neuronal mitochondria in their natural habitat, for revealing the influence that disease-related alterations have on this behavior and for testing pharmacological compounds and genetic manipulations that might ameliorate disease-related mitochondrial phenotypes in neurons.

Original languageEnglish
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages151-164
Number of pages14
EditionC
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
NumberC
Volume547
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Axonal transport
  • In vivo imaging
  • Microscopy
  • Zebrafish

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