In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography

Claudio Vinegoni, Chrysoula Pitsouli, Daniel Razansky, Norbert Perrimon, Vasilis Ntziachristos

Research output: Contribution to journalArticlepeer-review

104 Scopus citations

Abstract

We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.

Original languageEnglish
Pages (from-to)45-47
Number of pages3
JournalNature Methods
Volume5
Issue number1
DOIs
StatePublished - Jan 2008

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