TY - GEN
T1 - In vivo hybrid microscopy of small model organisms
AU - Seeger, Markus
AU - Westmeyer, Gil
AU - Ntziachristos, Vasilis
N1 - Publisher Copyright:
© SPIE-OSA 2019
PY - 2019
Y1 - 2019
N2 - We present the investigation of in vivo small model organisms, which are well established in biological and biomedical research, using a hybrid multiphoton and optoacoustic microscope (HyMPOM). The unique capabilities of HyMPOM for multimodal and potentially label-free signal acquisition, high resolution, as well as deep and fast imaging allow extraction of detailed information across large areas of living tissue on the microscale. Applying HyMPOM to living zebrafish-like fish larvae allowed exploration of the structural composition of the entire brain, including the brain vasculature and the neuronal network. Applying HyMPOM to the ears of living mice enabled accurate imaging of vasculature, connective tissue, keratinocytes, and sebaceous glands. The hybrid microscope proposed here constitutes a novel approach to explore small model organisms in vivo in great detail by revealing the spatial distribution and interplay of various tissue compartments on the microscale.
AB - We present the investigation of in vivo small model organisms, which are well established in biological and biomedical research, using a hybrid multiphoton and optoacoustic microscope (HyMPOM). The unique capabilities of HyMPOM for multimodal and potentially label-free signal acquisition, high resolution, as well as deep and fast imaging allow extraction of detailed information across large areas of living tissue on the microscale. Applying HyMPOM to living zebrafish-like fish larvae allowed exploration of the structural composition of the entire brain, including the brain vasculature and the neuronal network. Applying HyMPOM to the ears of living mice enabled accurate imaging of vasculature, connective tissue, keratinocytes, and sebaceous glands. The hybrid microscope proposed here constitutes a novel approach to explore small model organisms in vivo in great detail by revealing the spatial distribution and interplay of various tissue compartments on the microscale.
UR - http://www.scopus.com/inward/record.url?scp=85084487965&partnerID=8YFLogxK
U2 - 10.1117/12.2530923
DO - 10.1117/12.2530923
M3 - Conference contribution
AN - SCOPUS:85084487965
SN - 9781510628397
T3 - Optics InfoBase Conference Papers
BT - European Conference on Biomedical Optics, ECBO_2019
PB - Optica Publishing Group (formerly OSA)
T2 - European Conference on Biomedical Optics, ECBO_2019
Y2 - 23 June 2019 through 25 June 2019
ER -