In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging

Stefan Eser; Marlena Messer; Philipp Eser; Alexander Von Werder; Barbara Seidler; Monther Bajbouj; Roger Vogelmann; Alexander Meining; Johannes Von Burstin; Hana Algül; Philipp Pagel; Angelika E. Schnieke; Irene Esposito; Roland M. Schmid; Günter Schneider; Dieter Saur

doi:10.1073/pnas.1100890108

In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging

Stefan Eser, Marlena Messer, Philipp Eser, Alexander Von Werder, Barbara Seidler, Monther Bajbouj, Roger Vogelmann, Alexander Meining, Johannes Von Burstin, Hana Algül, Philipp Pagel, Angelika E. Schnieke, Irene Esposito, Roland M. Schmid, Günter Schneider, Dieter Saur

Technical University of Munich

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73 Scopus citations

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with poor patient outcome often resulting from late diagnosis in advanced stages. To date methods to diagnose early-stage PDAC are limited and in vivo detection of pancreatic intraepithelial neoplasia (PanIN), a preinvasive precursor of PDAC, is impossible. Using a cathepsin-activatable near-infrared probe in combination with flexible confocalfluorescence lasermicroscopy (CFL) in a genetically defined mouse model of PDAC we were able to detect and grade murine PanIN lesions in real time in vivo. Our diagnostic approach is highly sensitive and specific and proved superior to clinically established fluorescein-enhanced imaging. Translation of this endoscopic technique into the clinic should tremendously improve detection of pancreatic neoplasia, thus reforming management of patients at risk for PDAC.

Original language	English
Pages (from-to)	9945-9950
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	108
Issue number	24
DOIs	https://doi.org/10.1073/pnas.1100890108
State	Published - 14 Jun 2011

Keywords

Carcinoma in situ
Cathepsin
Early detection
Genetically engineered mouse model
Molecular in vivo imaging

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10.1073/pnas.1100890108

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Eser, S., Messer, M., Eser, P., Von Werder, A., Seidler, B., Bajbouj, M., Vogelmann, R., Meining, A., Von Burstin, J., Algül, H., Pagel, P., Schnieke, A. E., Esposito, I., Schmid, R. M., Schneider, G., & Saur, D. (2011). In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging. Proceedings of the National Academy of Sciences of the United States of America, 108(24), 9945-9950. https://doi.org/10.1073/pnas.1100890108

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title = "In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging",

abstract = "Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with poor patient outcome often resulting from late diagnosis in advanced stages. To date methods to diagnose early-stage PDAC are limited and in vivo detection of pancreatic intraepithelial neoplasia (PanIN), a preinvasive precursor of PDAC, is impossible. Using a cathepsin-activatable near-infrared probe in combination with flexible confocalfluorescence lasermicroscopy (CFL) in a genetically defined mouse model of PDAC we were able to detect and grade murine PanIN lesions in real time in vivo. Our diagnostic approach is highly sensitive and specific and proved superior to clinically established fluorescein-enhanced imaging. Translation of this endoscopic technique into the clinic should tremendously improve detection of pancreatic neoplasia, thus reforming management of patients at risk for PDAC.",

keywords = "Carcinoma in situ, Cathepsin, Early detection, Genetically engineered mouse model, Molecular in vivo imaging",

author = "Stefan Eser and Marlena Messer and Philipp Eser and {Von Werder}, Alexander and Barbara Seidler and Monther Bajbouj and Roger Vogelmann and Alexander Meining and {Von Burstin}, Johannes and Hana Alg{\"u}l and Philipp Pagel and Schnieke, {Angelika E.} and Irene Esposito and Schmid, {Roland M.} and G{\"u}nter Schneider and Dieter Saur",

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doi = "10.1073/pnas.1100890108",

language = "English",

volume = "108",

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Eser, S, Messer, M, Eser, P, Von Werder, A, Seidler, B, Bajbouj, M, Vogelmann, R, Meining, A, Von Burstin, J, Algül, H, Pagel, P, Schnieke, AE, Esposito, I, Schmid, RM, Schneider, G & Saur, D 2011, 'In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging', Proceedings of the National Academy of Sciences of the United States of America, vol. 108, no. 24, pp. 9945-9950. https://doi.org/10.1073/pnas.1100890108

In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging. / Eser, Stefan; Messer, Marlena; Eser, Philipp et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, No. 24, 14.06.2011, p. 9945-9950.

Research output: Contribution to journal › Article › peer-review

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T1 - In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging

AU - Eser, Stefan

AU - Messer, Marlena

AU - Eser, Philipp

AU - Von Werder, Alexander

AU - Seidler, Barbara

AU - Bajbouj, Monther

AU - Vogelmann, Roger

AU - Meining, Alexander

AU - Von Burstin, Johannes

AU - Algül, Hana

AU - Pagel, Philipp

AU - Schnieke, Angelika E.

AU - Esposito, Irene

AU - Schmid, Roland M.

AU - Schneider, Günter

AU - Saur, Dieter

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AB - Pancreatic ductal adenocarcinoma (PDAC) is a fatal disease with poor patient outcome often resulting from late diagnosis in advanced stages. To date methods to diagnose early-stage PDAC are limited and in vivo detection of pancreatic intraepithelial neoplasia (PanIN), a preinvasive precursor of PDAC, is impossible. Using a cathepsin-activatable near-infrared probe in combination with flexible confocalfluorescence lasermicroscopy (CFL) in a genetically defined mouse model of PDAC we were able to detect and grade murine PanIN lesions in real time in vivo. Our diagnostic approach is highly sensitive and specific and proved superior to clinically established fluorescein-enhanced imaging. Translation of this endoscopic technique into the clinic should tremendously improve detection of pancreatic neoplasia, thus reforming management of patients at risk for PDAC.

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KW - Early detection

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JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 24

ER -

In vivo diagnosis of murine pancreatic intraepithelial neoplasia and early-stage pancreatic cancer by molecular imaging

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