In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber

Helmuth Adelsberger, Christine Grienberger, Albrecht Stroh, Arthur Konnerth

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca2+ indicators, this optical fiber and its associated fluorescence detection system can be used for the in vivo recording of brain Ca2+ signals from a local cluster of coactive neurons. The fiber can also be used for the optogenetic stimulation of light-activated ion channels, such as channelrhodopsin-2, allowing the monitoring of local calcium signals evoked by optogenetic stimulation.

Original languageEnglish
Pages (from-to)1074-1079
Number of pages6
JournalCold Spring Harbor Protocols
Volume2014
Issue number10
DOIs
StatePublished - 1 Oct 2014

Fingerprint

Dive into the research topics of 'In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber'. Together they form a unique fingerprint.

Cite this