TY - JOUR
T1 - In vivo calcium recordings and channelrhodopsin-2 activation through an optical fiber
AU - Adelsberger, Helmuth
AU - Grienberger, Christine
AU - Stroh, Albrecht
AU - Konnerth, Arthur
N1 - Publisher Copyright:
© 2014 Cold Spring Harbor Laboratory Press.
PY - 2014/10/1
Y1 - 2014/10/1
N2 - We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca2+ indicators, this optical fiber and its associated fluorescence detection system can be used for the in vivo recording of brain Ca2+ signals from a local cluster of coactive neurons. The fiber can also be used for the optogenetic stimulation of light-activated ion channels, such as channelrhodopsin-2, allowing the monitoring of local calcium signals evoked by optogenetic stimulation.
AB - We describe here an approach for the fluorometric monitoring of population activity in neurons in live mice combined with the activation of optogenetic actuators in vivo. In this protocol, a thin multimode fiber, which is used for both delivering excitation light and collecting emitted fluorescence signals, is inserted into the skull of a mouse. When combined with multicell bolus loading of Ca2+ indicators, this optical fiber and its associated fluorescence detection system can be used for the in vivo recording of brain Ca2+ signals from a local cluster of coactive neurons. The fiber can also be used for the optogenetic stimulation of light-activated ion channels, such as channelrhodopsin-2, allowing the monitoring of local calcium signals evoked by optogenetic stimulation.
UR - http://www.scopus.com/inward/record.url?scp=84923872893&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot084145
DO - 10.1101/pdb.prot084145
M3 - Article
C2 - 25275110
AN - SCOPUS:84923872893
SN - 1940-3402
VL - 2014
SP - 1074
EP - 1079
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 10
ER -