In-vitro-untersuchungen zur wirkung homöopathischer potenzen von Thuja occidentalis an zellkulturen mit hilfe des MTT-testes

Translated title of the contribution: In vitro investigations on effects of homoeopathic potencies of Thuja occidentalis on cell cultures by use of the MTT assay

C. Then, J. Dittmann, A. Schütte, J. Bauer, G. Harisch

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Introduction: The vitality and activity of cells kept in cell cultures can be examined by the MTT assay using the catalytic activity of mitochondrial succinate dehydrogenase as indicator. The applicability of the MTT assay to clarify questions relevant for homoeopathy is studied. Objective: The main target of the investigations was to prove whether potencies of Thuja occidentalis influence the vitality and activity of cells kept in cell cultures. Methods: Potencies of Thuja occidentalis produced in water and cell medium as well as lactose potencies of this homoeopathic remedy were applied to adherent and immortalized EBL, HeLa and MDBK cells. After an incubation varying from 2 to 96 h the vitality of cells was determined by the MTT assay. Results: 1. With the described method effects of aqueous potencies can be observed up to D5, in some cases up to D6 as well. Using lactose potencies of Thuja occidentalis, effects were only found up to D3. 2. Stimulating as well as inhibiting effects were measured. There was a characteristic dynamic reaction in relation to the time of incubation. 3. There was a non-linear dose-effect response in potencies. This non-linear dose-effect relation was also observed in the morphological reaction of the cells. Conclusions: In principle, it is possible to use cells kept in cell cultures as indicators for effects caused by homoeopathically prepared substances.

Translated title of the contributionIn vitro investigations on effects of homoeopathic potencies of Thuja occidentalis on cell cultures by use of the MTT assay
Original languageGerman
Pages (from-to)280-287
Number of pages8
JournalForschende Komplementarmedizin und Klassische Naturheilkunde
Volume3
Issue number6
StatePublished - Dec 1996
Externally publishedYes

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