In vitro studies involving histamine and lysosomal enzyme release from human peripheral leukocytes with different human serum albumin (HSA) preparations

Various batches of differently prepared human serum albumin (HSA) were tested for their capacity to induce in vitro release of histamine or lysosomal enzymes from peripheral human leucocytes. While there was little effect of most of the native preparations tested, some batches showed significant release of histamine or lysosomal enzymes after heating to 70°C. HSA preparations produced by heat ethanol fractionation never induced significant release reactions. It is concluded that the mode of production and the selection of stabilizing agents might influence reactions of HSA with mediator-secreting cells.