In vitro Selection for Tolerance to Fusarium in F1 Microspore Populations of Wheat

F. Fadel, G. Wenzel

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Microspore populations of eight Fhybrids of winter wheat (Triticum aestivum L.) whose parents had different levels of resistance to Fusarium were screened in vitro, using phytotoxins of Fusarium as biochemical probe. Two selection methods were compared for the in vitro selection: either embryoids and calli were first initiated from anthers in toxin‐free medium and then grown on medium with 0.3—0.9 %Fusarium toxin; or anthers were immediately cultured in modified liquid potato‐2 medium in the presence of 1.5, 2.0 and 2.5ml Fusarium toxin per liter culture medium, a concentration which reduced the number of calli and embryoids to about 10 % compared to the toxin‐free controls. Microspores from donor hybrids which were produced from very susceptible cultivars were killed by lower toxin concentrations than micro‐spores from hybrids of less susceptible parents. From surviving calli and embryoids, originally initiated from 242,000 anthers in both procedures a total of 375 green lines could be regenerated. The results indicate that it is possible to enrich the fraction of regenerating microspores by those which contain the gene complex responsible for reduced susceptibility to Fusarium by the use of a pathotoxin.

Original languageEnglish
Pages (from-to)89-95
Number of pages7
JournalPlant Breeding
Volume110
Issue number2
DOIs
StatePublished - Mar 1993
Externally publishedYes

Keywords

  • Fusarium
  • Triticum aestivum
  • anther culture
  • haploid production
  • phytotoxins
  • resistance selection

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