In vitro Selection for Fusaric Acid Resistant Barley Plants

H. S. Chawla, G. Wenzel

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Calli of two genotypes of barley,‘Dissa’and W 193, were used for selection of resistance against fusaric acid, a pathotoxin of Fusarium. Callus was initiated from 7‐ to 10 days old immature embryos. 1000 calli of the‘Dissa’and 500 of the W 193 genotypes were grown for 4 selection cycles on medium with 0.8 mM fusaric acid. In the first selection cycle, about 80 % of the calli were killed; after the 4 selection cycles, 8 to 11 % resistant calli were obtained and plants were regenerated. Resistant calli maintained on non‐toxic medium showed retention of resistance ability after 3 months of sub‐culturing. Plants could be regenerated from the surviving calli and testing by leaf bioassay revealed that many were resistant to the same toxin concentration employed for callus selection (100 %), while some were only resistant up to a concentration of 75 %.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalPlant Breeding
Issue number2
StatePublished - Oct 1987
Externally publishedYes


  • Hardeum vulgare
  • callus‐derived plants
  • fusaric acid
  • in vitro selection toxin resistance


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