In vitro perfusion technique for investigations on the intestinal transport of water soluble substances

Gertrud Rehner; Hannelore Daniel; Renate Aeppli-Schmidt

doi:10.1016/0160-5402(81)90087-5

In vitro perfusion technique for investigations on the intestinal transport of water soluble substances

Gertrud Rehner
, Hannelore Daniel
, Renate Aeppli-Schmidt

Justus-Liebig-Universität Gießen

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A new method for the in vitro perfusion of isolated intestinal segments of laboratory animals is described. Everted intestinal segments are used in an "open perfusion system," and are incubated in a buffer solution containing the substrate to be absorbed as a radiolabeled compound. The solutes absorbed are removed from the serosal side in a buffer solution that is collected by a fraction collector. The technique permits studies on the mechanism and the kinetics of the intestinal transport of water soluble substances.

Original language	English
Pages (from-to)	193-201
Number of pages	9
Journal	Journal of Pharmacological Methods
Volume	5
Issue number	3
DOIs	https://doi.org/10.1016/0160-5402(81)90087-5
State	Published - May 1981
Externally published	Yes

Keywords

Everted intestinal segment
In vitro perfusion
Intestinal absorption
Transport kinetics
Transport mechanism

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10.1016/0160-5402(81)90087-5

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title = "In vitro perfusion technique for investigations on the intestinal transport of water soluble substances",

abstract = "A new method for the in vitro perfusion of isolated intestinal segments of laboratory animals is described. Everted intestinal segments are used in an {"}open perfusion system,{"} and are incubated in a buffer solution containing the substrate to be absorbed as a radiolabeled compound. The solutes absorbed are removed from the serosal side in a buffer solution that is collected by a fraction collector. The technique permits studies on the mechanism and the kinetics of the intestinal transport of water soluble substances.",

keywords = "Everted intestinal segment, In vitro perfusion, Intestinal absorption, Transport kinetics, Transport mechanism",

author = "Gertrud Rehner and Hannelore Daniel and Renate Aeppli-Schmidt",

note = "Funding Information: This work was supported in part by the Deutsche Forschungsgemeinschaft authors are grateful to E. L. Sattler for giving them the opportunity to work Radiation Center (Central Department) of the Justus-Liebig-University.",

year = "1981",

month = may,

doi = "10.1016/0160-5402(81)90087-5",

language = "English",

volume = "5",

pages = "193--201",

journal = "Journal of Pharmacological Methods",

issn = "0160-5402",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - In vitro perfusion technique for investigations on the intestinal transport of water soluble substances

AU - Rehner, Gertrud

AU - Daniel, Hannelore

AU - Aeppli-Schmidt, Renate

N1 - Funding Information: This work was supported in part by the Deutsche Forschungsgemeinschaft authors are grateful to E. L. Sattler for giving them the opportunity to work Radiation Center (Central Department) of the Justus-Liebig-University.

PY - 1981/5

Y1 - 1981/5

N2 - A new method for the in vitro perfusion of isolated intestinal segments of laboratory animals is described. Everted intestinal segments are used in an "open perfusion system," and are incubated in a buffer solution containing the substrate to be absorbed as a radiolabeled compound. The solutes absorbed are removed from the serosal side in a buffer solution that is collected by a fraction collector. The technique permits studies on the mechanism and the kinetics of the intestinal transport of water soluble substances.

AB - A new method for the in vitro perfusion of isolated intestinal segments of laboratory animals is described. Everted intestinal segments are used in an "open perfusion system," and are incubated in a buffer solution containing the substrate to be absorbed as a radiolabeled compound. The solutes absorbed are removed from the serosal side in a buffer solution that is collected by a fraction collector. The technique permits studies on the mechanism and the kinetics of the intestinal transport of water soluble substances.

KW - Everted intestinal segment

KW - In vitro perfusion

KW - Intestinal absorption

KW - Transport kinetics

KW - Transport mechanism

UR - https://www.scopus.com/pages/publications/0019495778

U2 - 10.1016/0160-5402(81)90087-5

DO - 10.1016/0160-5402(81)90087-5

M3 - Article

C2 - 7311559

AN - SCOPUS:0019495778

SN - 0160-5402

VL - 5

SP - 193

EP - 201

JO - Journal of Pharmacological Methods

JF - Journal of Pharmacological Methods

IS - 3

ER -

In vitro perfusion technique for investigations on the intestinal transport of water soluble substances

Abstract

Keywords

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