In Vitro and In Vivo Development of the Human Airway at Single-Cell Resolution

Alyssa J. Miller, Qianhui Yu, Michael Czerwinski, Yu Hwai Tsai, Renee F. Conway, Angeline Wu, Emily M. Holloway, Taylor Walker, Ian A. Glass, Barbara Treutlein, J. Gray Camp, Jason R. Spence

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

Miller et al. show that transient SMAD activation followed by inhibition patterns human lung bud tip progenitor organoids to functional airway cell types, including basal cells. Comparison of the organoids and the human lung epithelium developing in vivo by scRNA-seq demonstrates a high degree of transcriptomic similarity.

Original languageEnglish
Pages (from-to)117-128.e6
JournalDevelopmental Cell
Volume53
Issue number1
DOIs
StatePublished - 6 Apr 2020
Externally publishedYes

Keywords

  • basal cell
  • human development
  • lung
  • lung development
  • lung organoid
  • organoid
  • single-cell RNA-seq

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