TY - JOUR
T1 - In situ hybridization of cytospin preparations
T2 - A rapid nonisotopic screening method for isolated cells
AU - Mandry, Patricia
AU - Murray, A. Beatrice
AU - Höfler, Heinz
PY - 1994
Y1 - 1994
N2 - A simple and rapid method for light microscopic in situ hybridization on cytospin preparations is described and demonstrated for detection of viral nucleic acid in a virus-infected cell line. Cells were fixed by acetone followed by chloroform, denatured by heat, hybridized at 37 C, and hybridized sites detected with a multiple step procedure (primary anti-biotin antibody, biotinylated second antibody, streptavidin-peroxidase). This method can be used for screening studies at the light microscope level, and offers a useful and simple way to determine optimum hybridization conditions for subsequent electron microscopic investigations.
AB - A simple and rapid method for light microscopic in situ hybridization on cytospin preparations is described and demonstrated for detection of viral nucleic acid in a virus-infected cell line. Cells were fixed by acetone followed by chloroform, denatured by heat, hybridized at 37 C, and hybridized sites detected with a multiple step procedure (primary anti-biotin antibody, biotinylated second antibody, streptavidin-peroxidase). This method can be used for screening studies at the light microscope level, and offers a useful and simple way to determine optimum hybridization conditions for subsequent electron microscopic investigations.
KW - Biotin
KW - Cytospin
KW - In situ hybridization
KW - Light microscopy
KW - Nonisotopic screening
UR - http://www.scopus.com/inward/record.url?scp=0028241743&partnerID=8YFLogxK
U2 - 10.3109/10520299409106280
DO - 10.3109/10520299409106280
M3 - Article
C2 - 8068811
AN - SCOPUS:0028241743
SN - 1052-0295
VL - 69
SP - 165
EP - 170
JO - Biotechnic and Histochemistry
JF - Biotechnic and Histochemistry
IS - 3
ER -