In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

Stefan Juretschko; Wilhelm Schönhuber; Saulius Kulakauskas; Dusko S. Ehrlich; Karl Heinz Schleifer; Rudolf Amann

doi:10.1016/S0723-2020(99)80022-8

In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

Stefan Juretschko
, Wilhelm Schönhuber
, Saulius Kulakauskas
, Dusko S. Ehrlich
, Karl Heinz Schleifer
, Rudolf Amann

TUM Emeriti of Excellence

Max Planck Institute for Marine Microbiology
UMR System

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.

Original language	English
Pages (from-to)	1-8
Number of pages	8
Journal	Systematic and Applied Microbiology
Volume	22
Issue number	1
DOIs	https://doi.org/10.1016/S0723-2020(99)80022-8
State	Published - 1999

Keywords

ColE1-related plasmids
Escherichia coli
Fluorescence in situ hybridization
In situ detection
RNA I
RNA II

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10.1016/S0723-2020(99)80022-8

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title = "In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II",

abstract = "A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.",

keywords = "ColE1-related plasmids, Escherichia coli, Fluorescence in situ hybridization, In situ detection, RNA I, RNA II",

author = "Stefan Juretschko and Wilhelm Sch{\"o}nhuber and Saulius Kulakauskas and Ehrlich, \{Dusko S.\} and Schleifer, \{Karl Heinz\} and Rudolf Amann",

year = "1999",

doi = "10.1016/S0723-2020(99)80022-8",

language = "English",

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journal = "Systematic and Applied Microbiology",

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T1 - In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

AU - Juretschko, Stefan

AU - Schönhuber, Wilhelm

AU - Kulakauskas, Saulius

AU - Ehrlich, Dusko S.

AU - Schleifer, Karl Heinz

AU - Amann, Rudolf

PY - 1999

Y1 - 1999

N2 - A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.

AB - A method is described for the in situ detection of individual whole fixed cells of Escherichia coli containing ColE1-related plasmids. It makes use of fluorescence in situ hybridization (FISH) and the regulatory RNA II as a target molecule for both, Cy3- and HRP-labeled olinucleotide probes. Various methods for signal amplification were compared. Probes targeting the regulatory RNA I did not result in the in situ detection of plasmid-bearing cells.

KW - ColE1-related plasmids

KW - Escherichia coli

KW - Fluorescence in situ hybridization

KW - In situ detection

KW - RNA I

KW - RNA II

UR - https://www.scopus.com/pages/publications/0344889095

U2 - 10.1016/S0723-2020(99)80022-8

DO - 10.1016/S0723-2020(99)80022-8

M3 - Article

C2 - 10188273

AN - SCOPUS:0344889095

SN - 0723-2020

VL - 22

SP - 1

EP - 8

JO - Systematic and Applied Microbiology

JF - Systematic and Applied Microbiology

IS - 1

ER -

In situ detection of Escherichia coli cells containing ColE1-related plasmids by hybridization to regulatory RNA II

Abstract

Keywords

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