Improvement of constraint-based flux estimation during L-phenylalanine production with Escherichia coli using targeted knock-out mutants

Keyphrases

• Escherichia Coli 100%
• Phenylalanine 100%
• Constraint-based 100%
• Flux Estimation 100%
• Phenylalanine Production 100%
• Targeted Knockout 100%
• Batch Production 33%
• Malic Enzyme 33%
• Fed-batch 33%
• Malate 33%
• Knockout mutant 33%
• MaeA 33%
• Redundancy 16%
• Production Process 16%
• Process Performance 16%
• Carbon Source 16%
• Glycerol 16%
• Product Formation 16%
• Variability Analysis 16%
• Production Rate 16%
• Aromatic Amino Acids 16%
• Genetic Engineering 16%
• Flux Distribution 16%
• Anaplerotic Reactions 16%
• Degradation Reaction 16%
• Escherichia Coli Strains 16%
• Central Carbon Metabolism 16%
• L Scale 16%
• Central Metabolism 16%
• Recombinant Escherichia Coli 16%
• Recombinant Strain 16%
• Identical Processes 16%
• Flux Uncertainty 16%
• Flux Variability Analysis 16%
• Constraint Analysis 16%
• MaeB 16%

Biochemistry, Genetics and Molecular Biology

• Escherichia coli 100%
• Phenylalanine 100%
• Enzyme 33%
• Metabolic Pathway 16%
• Amino Acids 16%
• Glycerol 16%
• Carbon Metabolism 16%

Engineering

• Batch Manufacturing 100%
• Analyse Variability 100%
• Aromatics 50%
• Process Performance 50%