Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Ejvind Mortz; Thomas N. Krogh; Henrik Vorum; Angelika Görg

doi:10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q

Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Ejvind Mortz, Thomas N. Krogh, Henrik Vorum, Angelika Görg

TUM Emeriti of Excellence

Research output: Contribution to journal › Article › peer-review

475 Scopus citations

Abstract

In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

Original language	English
Pages (from-to)	1359-1363
Number of pages	5
Journal	Proteomics
Volume	1
Issue number	11
DOIs	https://doi.org/10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q
State	Published - Nov 2001

Keywords

Mass spectrometry
Peptide map
Protein identification
Silver stain

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10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q

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title = "Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis",

abstract = "In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.",

keywords = "Mass spectrometry, Peptide map, Protein identification, Silver stain",

author = "Ejvind Mortz and Krogh, {Thomas N.} and Henrik Vorum and Angelika G{\"o}rg",

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AU - Mortz, Ejvind

AU - Krogh, Thomas N.

AU - Vorum, Henrik

AU - Görg, Angelika

PY - 2001/11

Y1 - 2001/11

N2 - In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

AB - In proteomics it is essential to be able to detect proteins separated by gel electrophoresis at high sensitivity. Silver staining is currently the most popular method. Here we present silver staining protocols that are optimized for staining sensitivity, peptide recovery and compatibility with digestion and mass spectrometry.

KW - Mass spectrometry

KW - Peptide map

KW - Protein identification

KW - Silver stain

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U2 - 10.1002/1615-9861(200111)1:11<1359::AID-PROT1359>3.0.CO;2-Q

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ER -

Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis

Abstract

Keywords

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