Improved protocol for the extraction of bacterial mRNA from soils

Shilpi Sharma; Ravikumar Mehta; Rashi Gupta; Michael Schloter

doi:10.1016/j.mimet.2012.07.016

Improved protocol for the extraction of bacterial mRNA from soils

Shilpi Sharma, Ravikumar Mehta, Rashi Gupta, Michael Schloter

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.

Original language	English
Pages (from-to)	62-64
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	91
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2012.07.016
State	Published - Oct 2012
Externally published	Yes

Keywords

GlnA
MRNA
Soil

Access to Document

10.1016/j.mimet.2012.07.016

Cite this

@article{d4df306a4be640dbacbf0ef96e34f9ad,

title = "Improved protocol for the extraction of bacterial mRNA from soils",

abstract = "An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.",

keywords = "GlnA, MRNA, Soil",

author = "Shilpi Sharma and Ravikumar Mehta and Rashi Gupta and Michael Schloter",

year = "2012",

month = oct,

doi = "10.1016/j.mimet.2012.07.016",

language = "English",

volume = "91",

pages = "62--64",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Improved protocol for the extraction of bacterial mRNA from soils

AU - Sharma, Shilpi

AU - Mehta, Ravikumar

AU - Gupta, Rashi

AU - Schloter, Michael

PY - 2012/10

Y1 - 2012/10

N2 - An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.

AB - An improved protocol for extraction of prokaryotic mRNA from soil samples was developed by modifying the extraction procedure to obtain higher yields of mRNA and to reduce co-extraction of humic acids. The modified protocol was found to be more robust and efficient compared to the original protocol by Griffiths et al. (2000) without compromising with the quality and quantity of RNA.

KW - GlnA

KW - MRNA

KW - Soil

UR - http://www.scopus.com/inward/record.url?scp=84864816657&partnerID=8YFLogxK

U2 - 10.1016/j.mimet.2012.07.016

DO - 10.1016/j.mimet.2012.07.016

M3 - Article

C2 - 22841738

AN - SCOPUS:84864816657

SN - 0167-7012

VL - 91

SP - 62

EP - 64

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Improved protocol for the extraction of bacterial mRNA from soils

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this