Improved Enzyme Immunoassay for the Analysis of S-Triazines in Water Samples

Christine Wittmann, Bertold Hock

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

A highly sensitive enzyme immunoassay is described for the detection of the s-triazines, propazine and atrazine. Polyclonal antibodies were raised in rabbits by immunization with an atrazine-BSA conjugate containing 35 atrazine residues per molecule. A microtitration plate ELISA was optimized with a peroxidase-atrazine tracer. The middle of the test (50% B/B0) was found at 10 ng/l propazine and 20 ng/l atrazine, i.e. well below the maximum concentration permitted by the EC guidelines for drinking water. Detection limits for atrazine of about 1 ng/l and for propazine of around 0•1 ng/l could be reached. The assay did not require concentration or clean-up steps for drinking or ground water samples. Validation experiments showed a good accuracy and precision. Since propazine is not used in most European countries, this test can be applied to obtain fairly accurate results for atrazine concentrations in environmental water samples from these regions.

Original languageEnglish
Pages (from-to)211-224
Number of pages14
JournalFood and Agricultural Immunology
Volume1
Issue number4
DOIs
StatePublished - 1 Jan 1989
Externally publishedYes

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