Improved Culture System for Microspores of Barley to Become a Target for DNA Uptake

Ursula Kuhlmann, Bärbel Foroughi‐Wehr, A. Graner, G. Wenzel

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

For the winter barley cultivar ‘Igri’, a microspore isolation and regeneration procedure is described which allows the production of such vigorous micro‐spore fractions that this single‐cell system can be used as a target for DNA uptake. Up to 60 % of the vigorous microspores isolated from the anther, and cultured in liquid modified MS medium, formed embryoids and/or calli. Such preparations were used for trials in DNA uptake with the plasmid pBI 221. Transformation trials were performed with polyethylene glycol as the inducing agent. With this treatment, a relative increase of fluorescence could be shown under UV light indicative of transient expression of the uidA gene.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalPlant Breeding
Volume107
Issue number2
DOIs
StatePublished - Sep 1991
Externally publishedYes

Keywords

  • Hordeum vulgare
  • cell culture
  • haploid regeneration
  • microspores
  • transformation

Fingerprint

Dive into the research topics of 'Improved Culture System for Microspores of Barley to Become a Target for DNA Uptake'. Together they form a unique fingerprint.

Cite this